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嗜热和嗜温RNA聚合酶的冷敏感性。

Cold sensitivity of thermophilic and mesophilic RNA polymerases.

作者信息

Kulbachinskiy A, Bass I, Bogdanova E, Goldfarb A, Nikiforov V

机构信息

Institute of Molecular Genetics, Moscow, Russia.

出版信息

J Bacteriol. 2004 Nov;186(22):7818-20. doi: 10.1128/JB.186.22.7818-7820.2004.

DOI:10.1128/JB.186.22.7818-7820.2004
PMID:15516599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC524896/
Abstract

RNA polymerase from mesophilic Deinococcus radiodurans displays the same cold sensitivity of promoter opening as RNA polymerase from the closely related thermophilic Thermus aquaticus. This suggests that, contrary to the accepted view, cold sensitivity of promoter opening by thermophilic RNA polymerases may not be a consequence of their thermostability.

摘要

嗜温性耐辐射球菌的RNA聚合酶与亲缘关系相近的嗜热性水生栖热菌的RNA聚合酶一样，在启动子开放方面表现出相同的冷敏感性。这表明，与普遍观点相反，嗜热RNA聚合酶在启动子开放方面的冷敏感性可能并非其热稳定性的结果。

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本文引用的文献

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Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics.从比较基因组学角度看极端耐辐射细菌耐辐射球菌的基因组
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Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.嗜热酶：来源、用途及热稳定性的分子机制
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Recombinant Thermus aquaticus RNA polymerase, a new tool for structure-based analysis of transcription.重组嗜热栖热菌RNA聚合酶，一种用于基于结构的转录分析的新工具。
J Bacteriol. 2001 Jan;183(1):71-6. doi: 10.1128/JB.183.1.71-76.2001.
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Purification and initial characterization of RNA polymerase from Thermus thermophilus strain HB8.
Biochemistry. 2000 Nov 21;39(46):14356-62. doi: 10.1021/bi0012538.
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J Biol Chem. 2000 Feb 4;275(5):3661-6. doi: 10.1074/jbc.275.5.3661.
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