Ikegaya Hisato, Yamada Shin-Ya, Sonobe Seiji, Shimmen Teruo
Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo 678-1297, Japan.
J Plant Res. 2004 Dec;117(6):443-7. doi: 10.1007/s10265-004-0177-z. Epub 2004 Oct 28.
Some species of Spirogyra living in streams can anchor to the substratum by differentiating a rhizoid from a terminal cell of a filament. Rhizoid differentiation occurs in the light but not in the dark. When a filament of Spirogyra sp. competent for rhizoid differentiation was incubated in a medium containing 0.1% saponin, terminal cells were released one by one, forming single cells. Single cells effectively differentiated to be rhizoids when saponin in the incubation medium was removed. The single-cell system developed in the present study seems suitable for analysis of gene expression during rhizoid differentiation of Spirogyra.
一些生活在溪流中的水绵物种可以通过将丝状体的末端细胞分化为假根来附着在基质上。假根分化在光照条件下发生，而在黑暗中则不会发生。当将能够进行假根分化的水绵丝状体在含有0.1%皂苷的培养基中培养时，末端细胞会逐个释放，形成单细胞。当去除培养介质中的皂苷后，单细胞有效地分化为假根。本研究中建立的单细胞系统似乎适用于分析水绵假根分化过程中的基因表达。
