嘌呤蛋白（pura）与E2F-1转录因子之间的相互作用。
Interaction between the pura and E2F-1 transcription factors.
作者信息
Darbinian Nune, White Martyn K, Gallia Gary L, Amini Shohreh, Rappaport Jay, Khalili Kamel
机构信息
Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA.
出版信息
Anticancer Res. 2004 Sep-Oct;24(5A):2585-94.
BACKGROUND
Cell proliferation is regulated by E2F-1 which facilitates expression of genes involved in entry into S-phase. Release of E2F-1 from binding partners, e.g. pRb, is critical for G1/S progression. However ectopic E2F-1 overexpression activates p53 and inhibits growth. Previously, the multifunctional Pura protein was found to bind to E2F-1 and inhibit E2F-1 transcriptional activity.
MATERIALS AND METHODS
Pura deletion mutants were assayed for: in vitro binding to E2F-1, inhibition of E2F-1-induced promoter activation and effects on cell proliferation. Two RNA species with specific binding to E2F-1 and Pura were analyzed for their effects on E2F-1/Pura binding and cell growth.
RESULTS
The N-terminal 72 amino acids of Pura were involved in E2F-1 binding, inhibition of promoter activation by E2F-1 and reversal of E2F-mediated growth inhibition. The RNA species disrupted Pura/E2F-1 interaction and affected cell growth.
CONCLUSION
E2F-1/Pura interaction has a role in the control of cell proliferation.
背景
细胞增殖受E2F-1调控，E2F-1可促进参与进入S期的基因表达。E2F-1从结合伴侣（如pRb）上释放对于G1/S期进程至关重要。然而，异位E2F-1过表达会激活p53并抑制生长。此前，发现多功能Pura蛋白可与E2F-1结合并抑制E2F-1转录活性。
材料与方法
检测Pura缺失突变体在体外与E2F-1的结合、对E2F-1诱导的启动子激活的抑制作用以及对细胞增殖的影响。分析两种与E2F-1和Pura特异性结合的RNA物种对E2F-1/Pura结合及细胞生长的影响。
结果
Pura的N端72个氨基酸参与E2F-1结合、抑制E2F-1介导的启动子激活以及逆转E2F介导的生长抑制。RNA物种破坏了Pura/E2F-1相互作用并影响细胞生长。
结论
E2F-1/Pura相互作用在细胞增殖控制中起作用。
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