Fu S-H, Hsu B R-S, Juang J-H, Chen S-T, Yang T-Y, Hsu S
Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang-Gung Memorial Hospital, Taoyuan Hsien, Taiwan.
Transplant Proc. 2004 Sep;36(7):2205-6. doi: 10.1016/j.transproceed.2004.06.050.
To study the effect of treatment with cobalt-protoporphyrin (CoPP) for the induction of the heme oxygenase-1 (HO-1) enzyme on islet engraftment donor mice received either a single intraperitoneal injection of CoPP (20 mg/kg body weight) 1 day prior to islet isolation or this injection plus a 9 day posttransplantation course of Copp. After a single injection of CoPP, the CoPP-induced islets contained higher HO-1 proteins than did the normal islets both at 12 (5.3 +/- 1.5 vs 0.1 +/- 0.1 ng/mg protein, P < .01) and at 30 hours (6.8 +/- 2.1 vs 0.4 +/- 0.3 ng/mg protein, P < .05), but not at 56 hours (1.9 +/- 0.8 vs 1.6 +/- 0.8 ng/mg protein, P > .05). In contrast, diabetic mice that received 75 CoPP-induced islets and a 9-day CoPP injection course posttransplantation showed better improvement in blood glucose levels and body weights than did the mice that only received CoPP-induced islets. Mice of both CoPP-treated groups displayed better improvement in glycemic control than mice that received control islets. At 8 weeks after transplantation, the insulin content of grafts from both CoPP groups was significantly higher than that in the control group. In conclusion, CoPP treatment for the induction of HO-1 enhances engraftment of islets in a syngeneic murine transplantation model.
为研究用钴原卟啉（CoPP）诱导血红素加氧酶-1（HO-1）对胰岛移植的影响，供体小鼠在胰岛分离前1天接受单次腹腔注射CoPP（20mg/kg体重），或该注射加移植后9天的CoPP疗程。单次注射CoPP后，CoPP诱导的胰岛在12小时（5.3±1.5对0.1±0.1ng/mg蛋白，P<.01）和30小时（6.8±2.1对0.4±0.3ng/mg蛋白，P<.05）时所含HO-1蛋白高于正常胰岛，但在56小时时并非如此（1.9±0.8对1.6±0.8ng/mg蛋白，P>.05）。相比之下，接受75个CoPP诱导的胰岛并在移植后接受9天CoPP注射疗程的糖尿病小鼠，其血糖水平和体重的改善情况优于仅接受CoPP诱导的胰岛的小鼠。两个CoPP治疗组的小鼠在血糖控制方面的改善均优于接受对照胰岛的小鼠。移植后8周，两个CoPP组移植物中的胰岛素含量均显著高于对照组。总之，CoPP诱导HO-1的治疗可增强同基因小鼠移植模型中胰岛的移植。
