Suh Junghae, Dawson Michelle, Hanes Justin
Department of Biomedical Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore MD, 21218, USA.
Adv Drug Deliv Rev. 2005 Jan 2;57(1):63-78. doi: 10.1016/j.addr.2004.06.001.
Complex biological environments, such as the cell cytoplasm or the mucus lining the airways of the lungs, can pose significant barriers to efficient therapeutic drug and gene delivery. Biological barriers are particularly important in controlled drug delivery applications that utilize a large carrier particle, such as a liposome or a polymer micro- or nanosphere. The dynamic transport of particulate drug and gene delivery vehicles through these barriers is poorly understood, having been primarily studied with static methods in the past. Recently, the transport of synthetic drug and gene carriers has been investigated quantitatively with real-time particle tracking technology, providing new insight into particle behavior in complex biological environments that is guiding rational improvements in particle design. This review briefly highlights basic principles of particle tracking and its application to elucidate important phenomena that limit effective particulate drug and gene delivery.
复杂的生物环境，如细胞质或肺部气道内衬的黏液，会对高效治疗药物和基因递送构成重大障碍。在利用大载体颗粒（如脂质体或聚合物微球或纳米球）的控释药物递送应用中，生物屏障尤为重要。过去主要采用静态方法研究，对颗粒状药物和基因递送载体通过这些屏障的动态运输了解甚少。最近，已利用实时粒子跟踪技术对合成药物和基因载体的运输进行了定量研究，为复杂生物环境中粒子行为提供了新的见解，这正引导着粒子设计的合理改进。本综述简要强调了粒子跟踪的基本原理及其在阐明限制有效颗粒状药物和基因递送的重要现象方面的应用。
