防御素肽对真核细胞的影响：原代表皮细胞、成纤维细胞和鳞状细胞癌细胞系。
Effect of defensin peptides on eukaryotic cells: primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines.
作者信息
Nishimura Michiko, Abiko Yoshihiro, Kurashige Yoshihito, Takeshima Maiko, Yamazaki Mami, Kusano Kaoru, Saitoh Masato, Nakashima Keisuke, Inoue Takashi, Kaku Tohru
机构信息
Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
出版信息
J Dermatol Sci. 2004 Nov;36(2):87-95. doi: 10.1016/j.jdermsci.2004.07.001.
BACKGROUND
Although the usefulness of the antimicrobial peptides known as defensins has been suggested against oral and skin infections, possible adverse effects of the defensins on the host should be understood before clinical applications can be contemplated.
OBJECTIVE
In the present study, we investigated how alpha-defensin (HNP-1) and beta-defensins (hBD-1, -2, -3) affect cells including primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines, SCC-9 and KB.
METHOD
Cell proliferation was assessed by the direct cell counting and XTT assay.
RESULTS
We found that alpha-defensin promotes proliferation of the epithelial cells at low concentration but has a cytotoxic effect at high concentration. In contrast, beta-defensins have little effect on these cells at any concentration, suggesting that beta-defensins may have no adverse effects on the host.
CONCLUSION
Therefore, in terms of host response beta-defensins may be more suitable antimicrobial agents for clinical applications than alpha-defensins.
背景
尽管已表明被称为防御素的抗菌肽对口腔和皮肤感染有用，但在考虑临床应用之前，应了解防御素对宿主可能产生的不良反应。
目的
在本研究中，我们调查了α-防御素（HNP-1）和β-防御素（hBD-1、-2、-3）如何影响包括原代上皮细胞、成纤维细胞以及鳞状细胞癌细胞系SCC-9和KB在内的细胞。
方法
通过直接细胞计数和XTT分析评估细胞增殖。
结果
我们发现α-防御素在低浓度时促进上皮细胞增殖，但在高浓度时有细胞毒性作用。相比之下，β-防御素在任何浓度下对这些细胞几乎没有影响，这表明β-防御素可能对宿主没有不良反应。
结论
因此，就宿主反应而言，β-防御素可能比α-防御素更适合作为临床应用的抗菌剂。
Zotero 插件