单次注射脑源性神经营养因子可改变成年雄性大鼠下丘脑-垂体-肾上腺皮质轴的活性。
A single brain-derived neurotrophic factor injection modifies hypothalamo-pituitary-adrenocortical axis activity in adult male rats.
作者信息
Givalois Laurent, Naert Gaëlle, Rage Florence, Ixart Guy, Arancibia Sandor, Tapia-Arancibia Lucia
机构信息
Cerebral Plasticity Laboratory, FRE 2693 CNRS, University of Montpellier II, 34095 Montpellier, France.
出版信息
Mol Cell Neurosci. 2004 Nov;27(3):280-95. doi: 10.1016/j.mcn.2004.07.002.
Immobilization stress induces in adult male rats rapid activation of brain derived neurotrophic factor (BDNF) expression in the hypothalamic paraventricular nucleus (PVN) preceding the increases in corticotropin releasing hormone (CRH) and arginin-vasopressin (AVP) expression. The BDNF mRNA signal belatedly co-localizes with CRH and AVP mRNA signals in the PVN, as determined by in situ hybridization. Intracerebroventricular BDNF injections (5 microg/rat) in non-anesthetized adult male rats induce a gradual increase in the CRH mRNA signal whereas AVP mRNA signal progressively decreases in the parvocellular and magnocellular PVN portions. At the same time, the CRH hypothalamic content decreases while the AVP content increases. These variations are accompanied by increases in ACTH and corticosterone plasma concentrations. These results strongly suggest that BDNF could be a stress-responsive intercellular messenger since when it is exogenously administered acts as an important and early component in the activation and recruitment of hypothalamic CRH and AVP neurons.
制动应激在成年雄性大鼠中，会在促肾上腺皮质激素释放激素（CRH）和精氨酸加压素（AVP）表达增加之前，诱导下丘脑室旁核（PVN）中脑源性神经营养因子（BDNF）表达的快速激活。通过原位杂交确定，BDNF mRNA信号随后在PVN中与CRH和AVP mRNA信号共定位。在未麻醉的成年雄性大鼠中脑室内注射BDNF（5微克/只大鼠），会导致CRH mRNA信号逐渐增加，而在小细胞和大细胞PVN部分，AVP mRNA信号则逐渐减少。与此同时，下丘脑CRH含量降低，而AVP含量增加。这些变化伴随着促肾上腺皮质激素（ACTH）和皮质酮血浆浓度的升高。这些结果强烈表明，BDNF可能是一种应激反应性细胞间信使，因为当外源性给予BDNF时，它是激活和募集下丘脑CRH和AVP神经元的重要早期成分。
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