Zhao Suling, Nichols Jennifer, Smith Austin G, Li Meng
Institute for Stem Cell Research, University of Edinburgh, Edinburgh, EH9 3JQ, United Kingdom.
Mol Cell Neurosci. 2004 Nov;27(3):332-42. doi: 10.1016/j.mcn.2004.08.002.
Knowledge of lineage decision machinery in pluripotent embryonic stem (ES) cells may shed light on the process of germ layer segregation in the mammalian embryo and enable directed differentiation in vitro for biomedical applications. We have investigated the contribution of Class B1 Sox transcription factors to lineage choice during ES cell differentiation. We report that forced expression of Sox1 or Sox2 did not impair propagation of undifferentiated ES cells, but upon release from self-renewal promoted differentiation into neuroectoderm at the expense of mesoderm and endoderm. The efficient specification of a primary lineage by transcription factor manipulation provides a paradigm for instructing differentiation of ES cells for biopharmaceutical screening and cell therapy applications.
了解多能胚胎干细胞（ES细胞）中的谱系决定机制，可能有助于揭示哺乳动物胚胎中胚层分离的过程，并实现体外定向分化以用于生物医学应用。我们研究了B1类Sox转录因子在ES细胞分化过程中对谱系选择的作用。我们报告称，强制表达Sox1或Sox2不会损害未分化ES细胞的增殖，但在从自我更新状态释放后，会促进其分化为神经外胚层，同时牺牲中胚层和内胚层。通过转录因子操作有效指定主要谱系，为指导ES细胞分化以用于生物制药筛选和细胞治疗应用提供了一个范例。
