Huttunen S, Taipale T, Lappalainen N M, Kubin E, Lakkala K, Kaurola J
Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland.
Environ Pollut. 2005 Jan;133(2):315-26. doi: 10.1016/j.envpol.2004.05.033.
Pleurozium schreberi (Brid.) Mitt. and Hylocomium splendens (Hedw.) Schimp. samples from the Finnish Environmental Specimen Bank were used to study the UV-B-absorbing compounds as potential screens of the past radiation environment. The first series from southern and central Finland consisted of samples collected from 18 P. schreberi or H. splendens dominated sites in 1985 and 1995. The second series from four H. splendens dominated sites (two in southern and two in northern Finland) and two P. schreberi dominated sites (one southern and one northern) were from the years 1985, 1990, 1995 and 2000. In the first series, the moss surface area of the analyzed specimens (5 +/- 0.2 mg DW) was smaller in 1995 (40% of both species collected in June) than in 1985 (40% of P. schreberi and 55% of H. splendens collected in September). The methanol-extractable UV-B-absorbing compounds of P. schreberi in 1985 and 1995 measured as absorbance at 10 nm intervals at 280-360 nm per mg DW revealed a negative correlation with reconstructed UV-radiation data. In the second series, the UV-B-absorbing compounds per specific surface area (surface area mm2 per DW) showed significant differences between the years. The highest values in both species were obtained in 1990. In H. splendens, a difference between the southern and northern samples was observed. The preliminary study encouraged the use of environmental specimen bank samples to indicate changes in surface radiation conditions.
来自芬兰环境标本库的高山金发藓（Pleurozium schreberi (Brid.) Mitt.）和尖叶提灯藓（Hylocomium splendens (Hedw.) Schimp.）样本被用于研究吸收紫外线B的化合物，以此作为过去辐射环境的潜在筛选指标。芬兰南部和中部的第一组样本包括1985年和1995年从18个以高山金发藓或尖叶提灯藓为主的地点采集的样本。第二组样本来自四个以尖叶提灯藓为主的地点（芬兰南部两个，北部两个）和两个以高山金发藓为主的地点（南部一个，北部一个），采集年份为1985年、1990年、1995年和2000年。在第一组样本中，1995年被分析标本（5±0.2毫克干重）的苔藓表面积（6月采集的两种苔藓均为40%）小于1985年（9月采集的高山金发藓为40%，尖叶提灯藓为55%）。1985年和1995年高山金发藓中甲醇可提取的紫外线B吸收化合物，以每毫克干重在280 - 360纳米处以10纳米间隔的吸光度测量，与重建的紫外线辐射数据呈负相关。在第二组样本中，每单位比表面积（每干重的表面积平方毫米）的紫外线B吸收化合物在不同年份间存在显著差异。两种苔藓在1990年的值最高。在尖叶提灯藓中，观察到南部和北部样本之间存在差异。这项初步研究鼓励使用环境标本库样本来说明地表辐射条件的变化。
