Vivas J, Razquin B, López-Fierro P, Villena A J
Department of Cell Biology, Faculty of Biological and Environmental Sciences, University of León, 24071 León, Spain.
Fish Shellfish Immunol. 2005 Mar;18(3):223-33. doi: 10.1016/j.fsi.2004.07.005.
The Aeromonas hydrophila aroA is an attenuated strain that has been assessed as a live vaccine in rainbow trout, Oncorhynchus mykiss. In this study the effects of different culture media used to grow the strain on its survival after in vitro exposure to rainbow trout serum, and on its immunogenicity in rainbow trout were compared. Four culture media were tested: Luria broth (LB), Luria broth with 0.25% glucose, trypticase soy broth (TSB), and brain-heart infusion broth (BHIB). Bacteria grown in culture media with glucose (TSB, BHIB and LB with 0.25% glucose) showed reduced complement consumption and a lower serum susceptibility. O. mykiss vaccinated with inocula prepared with BHIB- and LB-grown aroA cells resuspended in phosphate-buffered saline (PBS) showed higher and longer-lasting serum agglutinating antibody titres than those vaccinated with TSB-grown bacteria. Thus, a direct relationship between serum resistance and immunogenicity could not be established, but BHIB and LB culture media were the most effective in increasing the immunogenicity of the A. hydrophila aroA vaccine.
嗜水气单胞菌aroA是一种减毒株，已被评估为虹鳟（Oncorhynchus mykiss）的活疫苗。在本研究中，比较了用于培养该菌株的不同培养基对其体外暴露于虹鳟血清后的存活率以及对虹鳟免疫原性的影响。测试了四种培养基：Luria肉汤（LB）、含0.25%葡萄糖的Luria肉汤、胰蛋白胨大豆肉汤（TSB）和脑心浸液肉汤（BHIB）。在含葡萄糖的培养基（TSB、BHIB和含0.25%葡萄糖的LB）中生长的细菌显示出补体消耗减少和血清敏感性降低。用重悬于磷酸盐缓冲盐水（PBS）中的BHIB和LB培养的aroA细胞制备的接种物对虹鳟进行疫苗接种后，其血清凝集抗体滴度高于用TSB培养的细菌接种的虹鳟，且持续时间更长。因此，无法建立血清抗性与免疫原性之间的直接关系，但BHIB和LB培养基在提高嗜水气单胞菌aroA疫苗的免疫原性方面最有效。
