使用离子液体对家蚕丝素蛋白进行溶解与再生

Dissolution and regeneration of Bombyx mori silk fibroin using ionic liquids.

作者信息

Phillips David M, Drummy Lawrence F, Conrady Deborah G, Fox Douglas M, Naik Rajesh R, Stone Morley O, Trulove Paul C, De Long Hugh C, Mantz Robert A

机构信息

Materials & Manufacturing Directorate, Air Force Research Laboratory, 2941 Hobson Way, Wright-Patterson AFB, Ohio 45433, USA.

出版信息

J Am Chem Soc. 2004 Nov 10;126(44):14350-1. doi: 10.1021/ja046079f.

DOI:10.1021/ja046079f

PMID:15521743

Abstract

In this work, the suitability of imidazolium-based ionic liquid solvents is investigated for the dissolution and regeneration of silkworm (Bombyx mori) silk. Within an ionic liquid the anion plays a larger role in dictating the ultimate solubility of the silk. The dissolution of the silk in the ionic liquid is confirmed using wide-angle X-ray scattering. The dissolved silk is also processed into 100 mum-thick, two-dimensional films, and the structure of these films is examined. The rinse solvent, acetonitrile or methanol, has a profound impact on both the topography of the films and the secondary structure of the silk protein. The image depicts a silkworm cocoon dissolved in 1-butyl-3-methylimidazolium chloride and then regenerated as a film with birefringence.

摘要

在这项工作中，研究了基于咪唑鎓的离子液体溶剂对家蚕（Bombyx mori）丝的溶解和再生的适用性。在离子液体中，阴离子在决定丝的最终溶解度方面起着更大的作用。使用广角X射线散射确认了丝在离子液体中的溶解。溶解的丝还被加工成100微米厚的二维薄膜，并对这些薄膜的结构进行了检查。冲洗溶剂乙腈或甲醇对薄膜的形貌和丝蛋白的二级结构都有深远影响。该图像描绘了一个溶解在1-丁基-3-甲基咪唑鎓氯化物中然后再生为具有双折射的薄膜的家蚕茧。