[脆性组氨酸三联体基因（FHIT）表达与喉鳞状细胞癌细胞增殖及转移的相关性]

[Correlation of FHIT expression to cell proliferation and metastasis of laryngeal squamous cell carcinoma].

作者信息

Yin De-Tao, Lu Xiu-Bo, Dong Ming-Min, Qiu Xin-Guang, Wang Qing-Zhao

机构信息

Department of General Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450 052, P.R. China.

出版信息

Ai Zheng. 2004 Nov;23(11):1338-41.

PMID:15522186

Abstract

BACKGROUND & OBJECTIVE: Fragile histidine triad (FHIT) gene, a candidate tumor suppressor gene, located at 3pl4.2, spans the most common fragile site, FRA3B, in human genomes. It is altered in many human cancers, such as head and neck squamous cell carcinoma. This study was to investigate correlation of FHIT expression to cell proliferation and metastasis of laryngeal squamous cell carcinoma (LSCC).

METHODS

Expression of FHIT protein, and proliferating cell nuclear antigen (PCNA) was detected by SP immunohistochemistry in 41 specimens of LSCC and their matched adjacent non-cancerous epithelium (NCE).

RESULTS

Positive rates of FHIT in NCE, and LSCC were 100.0% (41/41), and 46.3% (19/41) (P< 0.01); in LSCC of stage I-II, and stage III-IV were 69.6% (16/23), and 16.7% (3/18) (P< 0.01); in lymph node metastasis group, and no metastasis group were 20.0% (3/15), and 61.5% (16/26) (P< 0.05). PCNA labeling indexes in NCE, and LSCC were (9.98+/-2.34)%, and (50.71+/-13.64)% (P< 0.01). In LSCC, expression of FHIT negatively correlated with PCNA (P< 0.05).

CONCLUSION

Low expression of FHIT might play an important role in cell proliferation and metastasis of LSCC, FHIT might be a useful marker for evaluating biological behaviors of LSCC.

摘要

背景与目的

脆性组氨酸三联体（FHIT）基因是一种候选抑癌基因，位于3p14.2，跨越人类基因组中最常见的脆性位点FRA3B。它在许多人类癌症中发生改变，如头颈部鳞状细胞癌。本研究旨在探讨FHIT表达与喉鳞状细胞癌（LSCC）细胞增殖和转移的相关性。

方法

采用SP免疫组织化学法检测41例LSCC标本及其配对的癌旁正常上皮（NCE）中FHIT蛋白和增殖细胞核抗原（PCNA）的表达。

结果

FHIT在NCE和LSCC中的阳性率分别为100.0%（41/41）和46.3%（19/41）（P<0.01）；在Ⅰ-Ⅱ期和Ⅲ-Ⅳ期LSCC中的阳性率分别为69.6%（16/23）和16.7%（3/18）（P<0.01）；在有淋巴结转移组和无转移组中的阳性率分别为20.0%（3/15）和61.5%（16/26）（P<0.05）。PCNA标记指数在NCE和LSCC中分别为（9.98±2.34）%和（50.71±13.64）%（P<0.01）。在LSCC中，FHIT表达与PCNA呈负相关（P<0.05）。