Kang Chan, Shin Hyosul, Zhang Yongchao, Heller Adam
Department of Chemistry, Chonbuk National University, Chonju 561-756, Korea.
Bioelectrochemistry. 2004 Dec;65(1):83-8. doi: 10.1016/j.bioelechem.2004.08.001.
The "wired" bilirubin oxidase (BOD) bioelectrocatalyst is superior to pure platinum as an electrocatalyst of the four-electron electroreduction of O(2) to water. Not only is its overpotential for O(2) reduction lower, but unlike platinum, it is not affected by organic compounds like glucose. The "wired" BOD-coated carbon cathode operates for >1 week at 37 degrees C in a glucose-containing physiological buffer solution. One of its key applications would be in a glucose-O(2) biofuel cell, which would operate in living tissues. The cathode is, however, short-lived in serum, losing its electrocatalytic activity in a few hours. Here we show that the damaging serum component is a product of the reaction of urate and dissolved oxygen. Exclusion of urate, by application of Nafion film on the cathode, improves the stability in serum.
“有线”胆红素氧化酶（BOD）生物电催化剂作为将O₂四电子电还原为水的电催化剂，优于纯铂。它不仅O₂还原过电位更低，而且与铂不同，它不受葡萄糖等有机化合物的影响。“有线”BOD包覆的碳阴极在含葡萄糖的生理缓冲溶液中于37℃下可运行超过1周。其关键应用之一将是在可在活组织中运行的葡萄糖 - O₂生物燃料电池中。然而，该阴极在血清中寿命较短，几小时内就会失去电催化活性。在此我们表明，造成损害的血清成分是尿酸盐与溶解氧反应的产物。通过在阴极上涂覆Nafion膜排除尿酸盐，可提高在血清中的稳定性。
