Fernández Rubio E, Cuesta Rodríguez T
Laboratorio de Análisis Clínicos del Instituto Oftálmico, Departamento de Oftalmología, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Arch Soc Esp Oftalmol. 2004 Oct;79(10):485-91. doi: 10.4321/s0365-66912004001000004.
To ascertain the frequency and characteristics of chronic carriers of conjunctival pathogen bacteria among patients undergoing cataract surgery in our hospital, to allow the design of studies of their postsurgical endophthalmitis risk.
Retrospective study of the preoperative conjunctival flora of 784 patients operated on for cataracts in both eyes, in two operations separated by 213 days (SD 170), from November 1993 to December 1997. Results of both cultures for each patient were obtained from the Laboratory cataract preoperative database, by means of an auxiliary utility in dBASE-III-PLUS program. The preoperative bacteria in both surgeries were compared and the patients having the same pathogen bacteria (all except Staphylococcus coagulase negative and Corynebacterium sp.) were identified. The mean values and standard deviations were calculated using Epiinfo 6.04, and the Chi2 test was carried out using Excel 97.
The conjunctival flora stemming from the first preoperative culture of the 784 patients were statistically similar to those of our reference population. The pathogen bacteria decreased in the second preoperative culture; especially the Streptococcus Pneumoniae and Haemophilus sp. frequencies (p<0.05). The same pathogen was isolated in both cultures of 31 patients among the 784 who entered the study, Staphylococcus Aureus and Proteus Mirabilis being the most frequent colonizers. The mean age of those carriers was higher than the mean age of the reference population (p<0.01).
3.95% of our patients awaiting cataract surgery are usual conjunctival carriers of certain pathogen bacteria; this finding is associated with the age of the patients and possibly with some pre-existing diseases.
确定我院白内障手术患者中结膜病原菌慢性携带者的频率和特征，以便设计关于其术后眼内炎风险的研究。
对1993年11月至1997年12月期间接受双眼白内障手术的784例患者的术前结膜菌群进行回顾性研究，两次手术间隔213天（标准差170）。通过dBASE - III - PLUS程序中的辅助实用工具，从白内障术前实验室数据库获取每位患者的两次培养结果。比较两次手术的术前细菌情况，识别出具有相同病原菌（除凝固酶阴性葡萄球菌和棒状杆菌属外的所有细菌）的患者。使用Epiinfo 6.04计算均值和标准差，使用Excel 97进行卡方检验。
784例患者首次术前培养的结膜菌群在统计学上与我们的参考人群相似。第二次术前培养中病原菌减少；尤其是肺炎链球菌和嗜血杆菌属的频率（p<0.05）。在进入研究的784例患者中，31例患者的两次培养中分离出相同病原菌，金黄色葡萄球菌和奇异变形杆菌是最常见的定植菌。这些携带者的平均年龄高于参考人群的平均年龄（p<0.01）。
在我们等待白内障手术的患者中，3.95%是某些病原菌的常见结膜携带者；这一发现与患者年龄以及可能的一些基础疾病有关。
