Kim Soo-A, Ahn Sang-Gun, Kim Do Kyung, Kim Su Gwan, Lee Sang Ho, Kim Jin, Yoon Jung Hoon
Oral Biology Research Institute, BK 21 Project, Chosun University School of Dentistry, Gwangju, Korea.
In Vivo. 2004 Sep-Oct;18(5):609-14.
Although it is known that iNOS and COX-2 are abundantly expressed in oral premalignant and malignant lesions, respectively, the interaction between iNOS and COX-2 has not been extensively studied. The purpose of this study was to examine the alteration of the iNOS and COX-2 expression level during hamster buccal pouch (HBP) carcinogenesis.
The expression of both iNOS and COX-2 on normal, dysplastic mucosa and squamous cell carcinoma (SCC) from different differentiation stages in 7, 12-dimethylbenz[a]anthracene (DMBA)-induced HBP carcinogenesis was examined using immunohistochemical analysis.
The mean values of both iNOS and COX-2 expression increased gradually from control to dysplastic lesions and more to invasive SCC. The highest mean expression was SCC. The differences between both iNOS and COX-2 expression in the normal and that in the dysplastic and carcinoma lesions were statistically significant.
The results suggest that iNOS can enhance its ability to promote tumor growth in cooperation with COX-2. The expression of iNOS and COX-2 may be one of the factors that contribute to oral carcinogenesis.
尽管已知诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）分别在口腔癌前病变和恶性病变中大量表达，但iNOS与COX-2之间的相互作用尚未得到广泛研究。本研究的目的是检测仓鼠颊囊（HBP）致癌过程中iNOS和COX-2表达水平的变化。
采用免疫组织化学分析法检测7,12-二甲基苯并[a]蒽（DMBA）诱导的HBP致癌过程中不同分化阶段的正常、发育异常黏膜和鳞状细胞癌（SCC）中iNOS和COX-2的表达。
从对照组到发育异常病变，再到浸润性SCC，iNOS和COX-2表达的平均值逐渐升高。平均表达最高的是SCC。正常组织与发育异常及癌性病变中iNOS和COX-2表达的差异具有统计学意义。
结果表明，iNOS可与COX-2协同增强其促进肿瘤生长的能力。iNOS和COX-2的表达可能是导致口腔癌发生的因素之一。
