Seki Hiroko, Sei Yoshihisa, Shikii Kazuaki, Shimotakahara Sakurako, Utsumi Hiroaki, Yamaguchi Kentaro, Tashiro Mitsuru
Chemical Analysis Center, Chiba University, Yayoi, Inage, Chiba 263-8522, Japan.
Anal Sci. 2004 Oct;20(10):1467-70. doi: 10.2116/analsci.20.1467.
A difference diffusion-based NMR technique and cold-spray ionization mass spectrometry were employed as a solution-based approach for identifying a ligand binding with a protein receptor. The difference diffusion-based NMR technique, called difference NOE-pumping, can directly detect the ligand interacting with a protein receptor. This technique uses a simple pulse sequence and the diffusion filter can easily be optimized. The cold-spray ionization mass spectrometry (CSI-MS), a variant of electrospray ionization mass spectrometry (ESI-MS) operating at low temperature, has been applied to detect the ligand-receptor complex. The efficiency of these techniques for identifying binding ligands is demonstrated with the human serum albumin (HSA)-drug system.
一种基于差分扩散的核磁共振技术和冷喷雾电离质谱被用作一种基于溶液的方法来鉴定与蛋白质受体结合的配体。这种基于差分扩散的核磁共振技术，称为差分NOE泵浦，能够直接检测与蛋白质受体相互作用的配体。该技术使用简单的脉冲序列，并且扩散滤波器易于优化。冷喷雾电离质谱（CSI-MS）是在低温下运行的电喷雾电离质谱（ESI-MS）的一种变体，已被应用于检测配体-受体复合物。通过人血清白蛋白（HSA）-药物系统证明了这些技术在鉴定结合配体方面的效率。
