Zhang Jie, Shan Qing, Ma Ping, Jiang Yanming, Chen Peng, Wen Jingxia, Zhou You, Qian Huanwen, Pei Xuetao
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Sci China C Life Sci. 2004 Jun;47(3):241-50. doi: 10.1007/BF03182769.
Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bipolar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Electroretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas.
骨髓间充质干细胞（MSCs）能够分化为造血细胞系和间充质细胞系，但此前并不认为其参与视网膜的生成。在此我们报告，将骨髓间充质干细胞经视网膜下移植到正常或经Nd:YAG激光损伤的大鼠眼后，它们能够整合到视网膜色素上皮（RPE）层、光感受器层、双极细胞层和神经节细胞层。利用4',6-二脒基-2-苯基吲哚（DAPI）标记检测来追踪再填充细胞的起源。在移植后10、20、35和50天，用DAPI荧光来鉴定骨髓来源的视网膜细胞。未发现玫瑰花结形成，但在观察末期发现了一些随机分布的细胞。与正常视网膜相比，骨髓间充质干细胞来源的细胞在损伤视网膜中的扩散范围更广。免疫组织化学检测显示，尽管这些细胞能够表达神经元细胞核（NeuN）、神经元特异性烯醇化酶（NSE）、胶质纤维酸性蛋白（GFAP）和细胞角蛋白（CK），但与正常移植组相比，损伤移植组某些区域的蛋白表达存在异常。视网膜电图（ERG）显示，损伤移植组的ERG-b波显著高于两个激光损伤对照组。这些结果表明，一部分骨髓间充质干细胞在体内能够分化为类似视网膜的结构，且在正常和激光损伤的视网膜中分化情况有所不同。
