Shi Kerong, Wang Aiguo, Li Ning, Deng Xuemei
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China.
Sci China C Life Sci. 2004 Jun;47(3):287-92. doi: 10.1007/BF03182774.
Melanocortin receptor 1 (MC1R) gene, one of the important candidate genes for coat color trait, was used to analyze the single nucleotide polymorphism (SNP) in Chinese native pig breeds by PCR-single strand conformation polymorphism (PCR-SSCP). The study had also taken 3 imported pig breeds as control. The results showed that the three mutations G284A, T309C and T364C found in Chinese native pigs were consistent to the mutation found in the European Large Black individuals. However, 68CC or C492T and G728A were only found in the imported individuals, which were obviously different from the Chinese native pigs. Accordingly, we presumed that the coat colors of Chinese native pigs belonged to dominant black color system, which was completely distinct to that of imported pig breeds. Thus it was implied that MC1R gene was not the principal factor affecting the coat color differences of Chinese native pig breeds, but could be used to trace the molecular evolution of pig breeds.
黑素皮质素受体1（MC1R）基因是毛色性状的重要候选基因之一，采用聚合酶链反应-单链构象多态性（PCR-SSCP）技术分析中国地方猪种的单核苷酸多态性（SNP）。本研究还选取了3个引进猪种作为对照。结果表明，中国地方猪种中发现的G284A、T309C和T364C这三个突变与欧洲大黑猪个体中发现的突变一致。然而，68CC或C492T以及G728A仅在引进个体中发现，这与中国地方猪种明显不同。据此推测，中国地方猪种的毛色属于显性黑色系统，与引进猪种完全不同。因此，这暗示MC1R基因不是影响中国地方猪种毛色差异的主要因素，但可用于追踪猪种的分子进化。
