Du Aiying, Zhang Shangli, Miao Junying, Zhao Baoxiang
Institute of Developmental Biology, School of Life Sciences, Shandong University, Jinan, China.
Exp Lung Res. 2004 Sep;30(6):419-29. doi: 10.1080/01902140490476337.
3,4-(Methylenedioxy)-1-(2',3'-epoxypropyl)-benzene (safrole oxide) was synthesized in the authors' laboratory. To investigate the effects of safrole oxide on the growth and apoptosis of A549 human lung cancer cells, the authors treated the cells with safrole oxide, 112.36 to 449.44 micromol/L, for 24 to 48 hours. The results showed that the drug led A549 cells to apoptosis and blocked cell cycle completely at G1 phase and partly at G(2)-M phase. To further study the correlated mechanism, the authors examined P53 and H-Ras protein expressions by using immunofluorescence assay. They found that the expression of P53 was dramatically up-regulated but the expression of H-Ras was hardly affected by safrole oxide, 224.72 micromol/L, within 24 hours. Taken together, these results revealed that safrole oxide could induce apoptosis of A549 cells and suggested that safrole oxide might perform its function by blocking cells completely at G1 phase and partly at G(2)-M phase, and also by up-regulating the expression of P53 protein. These findings would raise exciting possibilities for cancer therapy in future.
3,4-亚甲二氧基-1-(2',3'-环氧丙基)-苯（黄樟素氧化物）是在作者的实验室合成的。为了研究黄樟素氧化物对A549人肺癌细胞生长和凋亡的影响，作者用112.36至449.44微摩尔/升的黄樟素氧化物处理细胞24至48小时。结果表明，该药物导致A549细胞凋亡，并使细胞周期在G1期完全阻滞，在G(2)-M期部分阻滞。为了进一步研究相关机制，作者采用免疫荧光分析法检测P53和H-Ras蛋白表达。他们发现，在24小时内，224.72微摩尔/升的黄樟素氧化物可使P53表达显著上调，但对H-Ras表达几乎没有影响。综上所述，这些结果表明黄樟素氧化物可诱导A549细胞凋亡，并提示黄樟素氧化物可能通过在G1期完全阻滞细胞、在G(2)-M期部分阻滞细胞以及上调P53蛋白表达来发挥其作用。这些发现将为未来的癌症治疗带来令人兴奋的可能性。
