Chirico G, Cannone F, Diaspro A, Bologna S, Pellegrini V, Nifosì R, Beltram F
INFM and Department of Physics, University of Milano Bicocca, Piazza della Scienza 3, 20126 Milano, Italy.
Phys Rev E Stat Nonlin Soft Matter Phys. 2004 Sep;70(3 Pt 1):030901. doi: 10.1103/PhysRevE.70.030901. Epub 2004 Sep 21.
Multi-photon driven photo-switching between dark and bright (fluorescent) states of a green fluorescent protein (GFP) mutant is demonstrated. A single-protein investigation shows the existence of two distinct bright states that display sharp two-photon cross-section bands peaked at 780 nm and at 870 nm. Fluorescence of these two species can be independently switched on and off. These results highlight a new photoconversion pathway for photochromic GFPs and can have significant applications in multi-photon confocal microscopy and in optical data-storage architectures.
已证明绿色荧光蛋白（GFP）突变体在暗态和亮态（荧光态）之间可通过多光子驱动进行光开关转换。单蛋白研究表明存在两种不同的亮态，它们显示出在780纳米和870纳米处达到峰值的尖锐双光子截面带。这两种状态的荧光可以独立地开启和关闭。这些结果突出了光致变色GFP的一种新的光转换途径，并且在多光子共聚焦显微镜和光学数据存储架构中可能具有重要应用。
