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冈比亚按蚊(双翅目:蚊科)DNA的复性动力学

Reassociation kinetics of Anopheles gambiae (Diptera: Culicidae) DNA.

作者信息

Besansky N J, Powell J R

机构信息

Department of Biology, Yale University, New Haven, Conn. 06511.

出版信息

J Med Entomol. 1992 Jan;29(1):125-8. doi: 10.1093/jmedent/29.1.125.

Abstract

The renaturation kinetics of nuclear DNA from the G3 colony of Anopheles gambiae Giles was studied to estimate the genome size and to determine the proportion of repeated sequences. An. gambiae has a haploid DNA content of 0.27 picograms or 2.6 x 10(8) basepairs. Analysis of reassociation kinetics indicated that the genome is composed of 61% single-copy and 33% repetitive sequences, with 6% foldback sequences.

摘要

研究了冈比亚按蚊吉尔斯G3菌落的核DNA复性动力学,以估计基因组大小并确定重复序列的比例。冈比亚按蚊的单倍体DNA含量为0.27皮克或2.6×10⁸个碱基对。复性动力学分析表明,基因组由61%的单拷贝序列和33%的重复序列组成,其中6%为回文序列。

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