Lukindu Martin, Love R Rebecca, Guelbeogo Moussa W, Small Scott T, Stephens Melissa T, Campbell Nathan R, Sagnon N'Fale, Costantini Carlo, Besansky Nora J
Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
Insects. 2020 Oct 13;11(10):693. doi: 10.3390/insects11100693.
Polymorphic chromosomal inversions have been implicated in local adaptation. In anopheline mosquitoes, inversions also contribute to epidemiologically relevant phenotypes such as resting behavior. Progress in understanding these phenotypes and their mechanistic basis has been hindered because the only available method for inversion genotyping relies on traditional cytogenetic karyotyping, a rate-limiting and technically difficult approach that is possible only for the fraction of the adult female population at the correct gonotrophic stage. Here, we focus on an understudied malaria vector of major importance in sub-Saharan Africa, . We ascertain and validate tag single nucleotide polymorphisms (SNPs) using high throughput molecular assays that allow rapid inversion genotyping of the three most common inversions at scale, overcoming the cytogenetic karyotyping barrier. These same inversions are the only available markers for distinguishing two ecotypes that differ in indoor resting behavior, Folonzo and Kiribina. Our new inversion genotyping tools will facilitate studies of ecotypic differentiation in and provide a means to improve our understanding of the roles of Folonzo and Kiribina in malaria transmission.
多态性染色体倒位与局部适应性有关。在按蚊中，倒位也与流行病学相关的表型有关，如静息行为。由于唯一可用的倒位基因分型方法依赖于传统的细胞遗传学核型分析，这是一种限速且技术难度大的方法，仅适用于处于正确生殖营养阶段的成年雌性群体中的一部分，因此在理解这些表型及其机制基础方面的进展受到了阻碍。在这里，我们关注的是撒哈拉以南非洲一种未被充分研究但具有重要意义的疟疾媒介。我们使用高通量分子检测方法确定并验证标签单核苷酸多态性（SNP），该方法能够大规模快速对三种最常见的倒位进行基因分型，克服了细胞遗传学核型分析的障碍。这些相同的倒位是区分两种在室内静息行为上不同的生态型（Folonzo和Kiribina）的唯一可用标记。我们新的倒位基因分型工具将促进对[该疟疾媒介名称]生态型分化的研究，并提供一种手段来增进我们对Folonzo和Kiribina在疟疾传播中作用的理解。
