在原核期选择胚胎进行第3天移植以及原核期冷冻保存可在新鲜周期和冷冻周期中获得较高的分娩率。
Selection of embryos for day-3 transfer at the pronuclear-stage and pronuclear-stage cryopreservation results in high delivery rates in fresh and frozen cycles.
作者信息
Hammitt Diane G, Sattler Christopher A, Manes Misty L, Singh Anita P
机构信息
Center for Reproductive Medicine, Mayo Clinic Scottsdale, Thunderbird Women's Health Center, 13737 North 92nd Street, Scottsdale, Arizona 85260, USA.
出版信息
J Assist Reprod Genet. 2004 Jul;21(7):271-8. doi: 10.1023/b:jarg.0000042013.62311.b2.
PURPOSE
Evaluate IVF-ET outcome data for a unique culture and cryopreservation strategy.
METHODS
Retrospective study of 92 patients. Embryos for day-3 transfer were selected at pronuclear-stage; all extra zygotes were cryopreserved at pronuclear-stage.
RESULTS
Delivery rates for Anonymous Oocyte Donation (Group I), patients <35 years (Group II), and 35-38 years (Group III) were 52.9%, 61.5%, and 51.7% for fresh and 38.5%, 33.3%, and 40.0% for frozen transfer. Deliveries per retrieval were 82.3%, 71.8%, and 58.6%. Only 0.88, 0.80, and 0.61 more zygotes were cultured than what were used for fresh transfer. Singleton, twin, and triplet rates were 64.6%, 31.2%, and 4.2% for fresh and 69.2%, 30.8%, and 0% for frozen.
CONCLUSIONS
Selection of day-3 transfer embryos at the pronuclear-stage and cryopreservation of extra zygotes results in high delivery rates in fresh and frozen cycles. This approach optimizes deliveries per retrieval and provides many patients with more than one pregnancy per retrieval.
目的
评估一种独特的培养和冷冻保存策略下的体外受精-胚胎移植（IVF-ET）结局数据。
方法
对92例患者进行回顾性研究。选择原核期胚胎进行第3天移植；所有多余的受精卵在原核期进行冷冻保存。
结果
匿名卵母细胞捐赠组（I组）、年龄<35岁患者组（II组）和35-38岁患者组（III组）新鲜移植的分娩率分别为52.9%、61.5%和51.7%，冷冻移植的分娩率分别为38.5%、33.3%和40.0%。每次取卵后的分娩率分别为82.3%、71.8%和58.6%。培养的受精卵仅比用于新鲜移植的多0.88、0.80和0.61个。新鲜移植的单胎、双胎和三胎率分别为64.6%、31.2%和4.2%，冷冻移植的分别为69.2%、30.8%和0%。
结论
在原核期选择第3天移植的胚胎并冷冻保存多余的受精卵，可使新鲜和冷冻周期的分娩率较高。这种方法优化了每次取卵后的分娩情况，并使许多患者每次取卵能获得不止一次妊娠。
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