Haraldsson B S, Johnsson E K, Rippe B
Department of Physiology and Nephrology, University of Göteborg, Sweden.
Kidney Int. 1992 Feb;41(2):310-6. doi: 10.1038/ki.1992.43.
Orosomucoid, or alpha 1-acid glycoprotein, a serum protein known to be an "acute phase reactant" has recently been shown to be needed for the maintenance of normal capillary permeability in skeletal muscle and mesentery. Therefore, we were interested in studying whether the glomerular capillary wall is affected by orosomucoid as well. For this purpose, left and right kidneys from nine rats (group A) were isolated and perfused in situ and in parallel using separate solutions of human albumin (1.8% in Tyrode), differing in their content of orosomucoid, one containing 0.21 g/liter, the other less than 0.005 g/liter. The temperature was kept at 8 degrees C in order to minimize tubular reabsorption of fluid and albumin. The two kidneys showed identical and stable vascular resistances during the experiments. Also the glomerular filtration rates (GFR) were stable between 30 and 33 ml/min/100 g kidney. Initially, the two kidneys showed similar fractional albumin clearance (theta) values of approximately 0.003. However, in the "absence" of orosomucoid theta increased to become four- to fivefold higher in the test kidney than in the control kidney at the end of the 1 1/2 hour experiment. This difference was observed in all rats, suggesting that orosomucoid is needed also for the maintenance of the glomerular permselectivity. In a separate group of eight animals (group B), orosomucoid-containing albumin solutions were used in parallel with horse serum solutions to perfuse the two kidneys of each rat, at 8 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)
血清类黏蛋白，即α1-酸性糖蛋白，一种已知的“急性期反应物”血清蛋白，最近被证明是维持骨骼肌和肠系膜正常毛细血管通透性所必需的。因此，我们有兴趣研究血清类黏蛋白是否也会影响肾小球毛细血管壁。为此，从9只大鼠（A组）中分离出左肾和右肾，在原位并行灌注，使用不同血清类黏蛋白含量的人白蛋白溶液（在泰罗德溶液中为1.8%），一种含有0.21克/升，另一种低于0.005克/升。温度保持在8摄氏度，以尽量减少液体和白蛋白的肾小管重吸收。实验过程中，两只肾脏的血管阻力相同且稳定。肾小球滤过率（GFR）也稳定在30至33毫升/分钟/100克肾脏之间。最初，两只肾脏的白蛋白清除分数（θ）值相似，约为0.003。然而，在“缺乏”血清类黏蛋白的情况下，在1个半小时的实验结束时，测试肾脏中的θ增加，比对照肾脏高出四到五倍。在所有大鼠中都观察到了这种差异，表明维持肾小球的选择通透性也需要血清类黏蛋白。在另一组8只动物（B组）中，在8摄氏度下，将含血清类黏蛋白的白蛋白溶液与马血清溶液并行用于灌注每只大鼠的两只肾脏。（摘要截取自250字）
