Raut Narendra M, Jaison P G, Aggarwal Suresh K
Fuel Chemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
J Chromatogr A. 2004 Oct 15;1052(1-2):131-6. doi: 10.1016/j.chroma.2004.08.054.
Separation and determination of lanthanides, Th and U is of great relevance in different fields of science and technology. Reversed-phase high-performance liquid chromatography (RP-HPLC) using alpha-hydroxy isobutyric acid (alpha-HIBA) as an eluent on reversed-phase column modified to cation exchanger has been reported to achieve the separation. However, under those conditions, Th and U are eluted amongst lanthanides, making their quantification difficult due to overlapping with some of the lanthanides peaks. In this work, different chromatographic parameters (concentrations of eluent and ion interaction reagent, pH, etc.) were studied systematically to arrive at optimum chromatographic conditions. Using the dual (concentration and pH) gradient conditions, lanthanides, Th and U could be separated in 11 min by RP-HPLC with sequential elution of Th and U after the elution of all the lanthanides. The separation methodology was tested using SY-3 rock sample for the separation and determination of lanthanides, Th and U. The method allows an accurate determination of these elements in a single run using a single column. Also, the method is fast and cost-effective compared to the reported methods.
镧系元素、钍和铀的分离与测定在不同的科学技术领域具有重大意义。据报道，在改性为阳离子交换剂的反相柱上，以α-羟基异丁酸（α-HIBA）为洗脱剂的反相高效液相色谱（RP-HPLC）可实现分离。然而，在这些条件下，钍和铀在镧系元素中被洗脱，由于与一些镧系元素峰重叠，使得它们的定量分析变得困难。在这项工作中，系统研究了不同的色谱参数（洗脱剂和离子相互作用试剂的浓度、pH值等），以获得最佳的色谱条件。采用双（浓度和pH）梯度条件，通过RP-HPLC可在11分钟内分离镧系元素、钍和铀，所有镧系元素洗脱后钍和铀依次洗脱。使用SY-3岩石样品对分离方法进行了测试，用于镧系元素、钍和铀的分离与测定。该方法允许使用单一色谱柱在一次运行中准确测定这些元素。此外，与已报道的方法相比，该方法快速且具有成本效益。
