Onimaru Hiroshi, Arata Akiko, Arata Satoru, Shirasawa Senji, Cleary Michael L
Department of Physiology, Showa University School of Medicine, Toyo 142-8555, Japan.
Brain Res Dev Brain Res. 2004 Nov 25;153(2):275-9. doi: 10.1016/j.devbrainres.2004.09.007.
To clarify the neuronal organization of the respiratory center of the mouse, we analyzed the spatio-temporal pattern of respiratory neuron activity in the ventral medulla of a newborn mouse preparation, using optical recordings. We also demonstrated optical images of the respiratory activity of two different lines of knock-out mice (Tlx3-/-, Pbx3-/-) that exhibit respiratory failure leading to neonatal death from dysfunction of central respiratory neuron activity. In the wild type mice, the respiratory neuron activity in the para-facial region of the rostral medulla appeared prior to inspiratory activity in the more caudal ventrolateral medulla. This rostral to caudal activity pattern was basically preserved in Tlx3-/- mice though the activity was more dispersed and weaker than in the wild type mice. Such an activity pattern was not clearly detected in Pbx3-/- mouse preparations. The difference in the spatio-temporal pattern between Tlx3-/- and Pbx3-/- suggests different levels of functional disorder of the respiratory center.
为阐明小鼠呼吸中枢的神经元组织，我们利用光学记录分析了新生小鼠制备物延髓腹侧呼吸神经元活动的时空模式。我们还展示了两种不同品系的基因敲除小鼠（Tlx3-/-、Pbx3-/-）呼吸活动的光学图像，这些小鼠因中枢呼吸神经元活动功能障碍而出现呼吸衰竭，导致新生儿死亡。在野生型小鼠中，延髓头端旁面部区域的呼吸神经元活动先于更尾端腹外侧延髓的吸气活动出现。这种从头端到尾端的活动模式在Tlx3-/-小鼠中基本保留，尽管其活动比野生型小鼠更分散、更弱。在Pbx3-/-小鼠制备物中未明确检测到这种活动模式。Tlx3-/-和Pbx3-/-之间时空模式的差异表明呼吸中枢功能障碍的程度不同。
