Khatter J C, Hoeschen R J, Dhalla N S
Res Commun Chem Pathol Pharmacol. 1979 Apr;24(1):57-66.
The effects of sodium pentobarbital on ATPase, Ca2+ binding and adenylate cyclase activities of the rat heart sarcolemmal preparations were investigated. The Na+ - K+ ATPase activity was diminished by pentobarbital in concentrations as low as 0.3 mM. Mg2+ ATPase, Ca2+ ATPase and adenylate cyclase activities were also depressed but required 10 to 15 mM of this agent. Pentobarbital in concentrations of 10 mM, however, caused an increase in sarcolemmal Ca2+ binding. Mitochondrial and microsomal ATPase activities were decreased by 1 and 5 mM concentrations of pentobarbital respectively, while myofibrillar ATPase activity was unaltered even at a concentration of 20 mM. These data suggest that cardiodepressant effects of high doses of pentobarbital may partly be explained on the basis of its actions on heart sarcolemma.
研究了戊巴比妥钠对大鼠心脏肌膜制剂中ATP酶、Ca2+结合及腺苷酸环化酶活性的影响。低至0.3 mM浓度的戊巴比妥钠即可降低Na+-K+ATP酶活性。Mg2+ATP酶、Ca2+ATP酶及腺苷酸环化酶活性也受到抑制，但需要10至15 mM的该药物浓度。然而，10 mM浓度的戊巴比妥钠可使肌膜Ca2+结合增加。1 mM和5 mM浓度的戊巴比妥钠分别降低了线粒体和微粒体ATP酶活性，而即使在20 mM浓度下，肌原纤维ATP酶活性也未改变。这些数据表明，高剂量戊巴比妥钠的心脏抑制作用部分可基于其对心脏肌膜的作用来解释。
