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In situ delivery of passive immunity by lactobacilli producing single-chain antibodies.通过产生单链抗体的乳酸杆菌原位传递被动免疫。
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抗轮状病毒VP4外衣壳蛋白VP8*亚基单链抗体的筛选及其在干酪乳杆菌中的表达

Selection of single-chain antibodies against the VP8* subunit of rotavirus VP4 outer capsid protein and their expression in Lactobacillus casei.

作者信息

Monedero Vicente, Rodríguez-Díaz Jesús, Viana Rosa, Buesa Javier, Pérez-Martínez Gaspar

机构信息

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Burjassot, Spain.

出版信息

Appl Environ Microbiol. 2004 Nov;70(11):6936-9. doi: 10.1128/AEM.70.11.6936-6939.2004.

DOI:10.1128/AEM.70.11.6936-6939.2004
PMID:15528568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC525132/
Abstract

Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

摘要

通过噬菌体展示技术分离出了识别轮状病毒外衣壳VP8*部分并在体外阻断轮状病毒感染的单链抗体（scFv）。构建了其中一种scFv在干酪乳杆菌中进行细胞外表达的载体。干酪乳杆菌能够将活性scFv分泌到生长培养基中，这表明益生菌有潜力被改造以表达适合体内抗轮状病毒治疗的分子。