He Qiang, Sanford Robert A
Department of Civil and Environmental Engineering, University of Illinois at Urbana/Champaign, Urbana, Illinois 61801-2352, USA.
Appl Environ Microbiol. 2004 Nov;70(11):6940-3. doi: 10.1128/AEM.70.11.6940-6943.2004.
Acetate threshold concentrations were determined under chlororespiring and Fe(III)-reducing conditions for Anaeromyxobacter dehalogenans strain 2CP-C. The acetate threshold concentrations measured were 69 +/- 4, 19 +/- 8, and <1 nM for chlororespiration, amorphous Fe(III) reduction, and Fe(III) citrate reduction, respectively. Residual DeltaG values of -75.4 kJ/mol of electrons for chlororespiration and -41.5 kJ/mol of electrons for amorphous Fe(III) reduction were calculated at the acetate threshold concentration. By comparing threshold concentrations for different metabolisms in a single organism, this study provides insight into the metabolic use of energy under different growth conditions.
测定了脱卤厌氧粘细菌2CP-C菌株在氯呼吸和铁(III)还原条件下的乙酸盐阈值浓度。测得的氯呼吸、无定形铁(III)还原和柠檬酸铁还原的乙酸盐阈值浓度分别为69±4、19±8和<1 nM。在乙酸盐阈值浓度下,计算出氯呼吸的电子剩余ΔG值为-75.4 kJ/mol电子,无定形铁(III)还原的电子剩余ΔG值为-41.5 kJ/mol电子。通过比较单一生物体中不同代谢的阈值浓度,本研究深入了解了不同生长条件下能量的代谢利用情况。