Ito H, Miyazaki M, Udagawa I, Koshikawa H, Okui K
First Department of Surgery, Chiba University, School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1992 Feb;93(2):144-9.
The changes of hepatic lysosomal enzymes and the hepatic cellular damage were investigated in rats with obstructive jaundice, phospholipase A2 (PL-A2) which is a strong labilizer of lysosomal membrane was added in the lysosomal fraction of rat's liver with various concentration. The activities of cathepsin D and beta-glucuronidase those were released by PL-A2 from lysosomal fraction were measured. The values of both lysosomal enzyme activities showed positive relation to the concentration of PL-A2, and were remarkably increased in obstructive jaundiced rats than in normal rats. We also measured the activity of cathepsin D released by Triton X-100 from lysosomal fraction of normal and jaundiced rat liver. The amount of lysosomal enzyme was more increased in obstructive jaundiced liver than in normal liver. Fragility score as the indicator for lysosomal membranous fragility was calculated as the ratio of cathepsin D released by PL-A2 to that released by Triton X-100. Fragility score was more increased in obstructive jaundiced rats than in normal rats. In conclusion, these data suggest that the fragility of lysosomal membrane could be enhanced in obstructive jaundiced liver.
研究了梗阻性黄疸大鼠肝脏溶酶体酶的变化及肝细胞损伤情况，将作为溶酶体膜强稳定剂的磷脂酶A2（PL - A2）以不同浓度加入大鼠肝脏的溶酶体组分中。测定了PL - A2从溶酶体组分释放的组织蛋白酶D和β - 葡萄糖醛酸酶的活性。两种溶酶体酶活性的值与PL - A2的浓度呈正相关，且在梗阻性黄疸大鼠中比正常大鼠显著增加。我们还测定了Triton X - 100从正常和黄疸大鼠肝脏溶酶体组分释放的组织蛋白酶D的活性。梗阻性黄疸肝脏中溶酶体酶的量比正常肝脏增加得更多。作为溶酶体膜脆性指标的脆性评分计算为PL - A2释放的组织蛋白酶D与Triton X - 100释放的组织蛋白酶D的比值。梗阻性黄疸大鼠的脆性评分比正常大鼠增加得更多。总之，这些数据表明梗阻性黄疸肝脏中溶酶体膜的脆性可能增强。
