Witek B, Ochwanowska E, Kolataj A, Slewa A, Stanislawska I
Department of Genetics, Institute of Biology, Swietokrzyska Academy, Poland.
Neuro Endocrinol Lett. 2001 Jun;22(3):181-5.
Changes in the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, cathepsin D and L, alanine aminopeptidase and lysosomal acid lipase in lysosomal fractions of the liver and kidneys of mice, which were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxytryptamine) for 7 and 14 days were investigated.
The slices of the liver and kidney were homogenized in 0.1M phosphate buffer, pH 7.0. Homogenates were subjected to differentiated centrifuging and determination of studied enzymes.
Melatonin caused lowering of the activity of all the investigated lysosomal enzymes in the liver and kidney.
Administration of melatonin was caused the lowering of the activity of the investigated lysosomal enzymes in comparison with values in control groups.
研究给小鼠腹腔注射20mg/kg体重的外源性褪黑素（N-乙酰-5-甲氧基色胺）7天和14天后，其肝脏和肾脏溶酶体组分中β-葡萄糖醛酸酶、N-乙酰-β-氨基葡萄糖苷酶、组织蛋白酶D和L、丙氨酸氨基肽酶及溶酶体酸性脂肪酶活性的变化。
将肝脏和肾脏切片在pH 7.0的0.1M磷酸盐缓冲液中匀浆。匀浆经差速离心后测定所研究的酶。
褪黑素使肝脏和肾脏中所有所研究的溶酶体酶活性降低。
与对照组相比，褪黑素给药导致所研究的溶酶体酶活性降低。
