Cojocaru Inimioara Mihaela, Cojocaru M, Muşuroi Camelia, Botezat Mihaela, Lazăr Livia, Drută Alina
Carol Davila University of Medicine and Pharmacy, Colentina Clinic of Neurology, Bucharest, Romania.
Rom J Intern Med. 2004;42(2):423-9.
Production of reactive oxygen species (ROS) increased in diabetic patients and oxidative damage may contribute to the development of diabetic complications. Malondialdehyde is known as marker of the oxidative damage. Catalase is one of antioxidative factors involved in elimination of ROS In this study, the plasma level of lipid peroxides and plasma catalase activity in 315 patients with diabetes mellitus were assayed. We also included 114 non-diabetic healthy controls whose age, sex were matched to the diabetic patients. The plasma levels of lipid peroxides (LPO) were determined by spectrophotometric method modified by Satoh and Yagi. Lipid peroxidation was estimated by the plasma level of malondialdehyde (MDA). In controls mean value of plasma lipid peroxides was 1.329 +/- 0.118 nmol/ml. In diabetic patients with ischemic stroke MDA level was 2.919 +/- 0.182 nmol/l; p<0.001, and in patients without ischemic stroke the MDA level was 2.329 +/- 0.149 nmol/l; p<0.05; between diabetic patients with ischemic stroke and patients without ischemic stroke p<0.01. The high level of lipid peroxides might induce a self-maintained chronic process which, in time, might lead to the aggravation of the macro- and microangiopathy in diabetes. The plasma levels of catalase were determined by Goth's spectrophotometric method. In 114 healthy persons the mean value of plasma catalase (CAT) activity was 115.3 +/- 14.5 MU/l with less plasma catalase for females (108.7 +/- 12.4 MU/l) than for males (118.9 +/- 16.6 MU/l). Mean value of plasma CAT was (102.4 +/- 12.7 MU/l in patients with ischemic stroke, p<0.001 and 116.3 +/- 18.7 MU/l in patients without ischemic stroke, p<0.05); between diabetic patients with ischemic stroke and patients without ischemic stroke p<0.01. Our results revealed a decrease in plasma CAT activity in patients with diabetes mellitus and ischemic stroke as compared to patients with diabetes mellitus without ischemic stroke. We can conclude that in diabetic patients the decrease in plasma CAT activity is the consequence of oxidative modifications. These results suggest that diabetic patients have significantly increased oxidative damage.
糖尿病患者体内活性氧（ROS）的生成增加，氧化损伤可能促使糖尿病并发症的发生。丙二醛是氧化损伤的标志物。过氧化氢酶是参与清除ROS的抗氧化因子之一。在本研究中，我们检测了315例糖尿病患者的血浆脂质过氧化物水平和血浆过氧化氢酶活性。我们还纳入了114名年龄、性别与糖尿病患者相匹配的非糖尿病健康对照者。脂质过氧化物（LPO）的血浆水平采用Satoh和Yagi改良的分光光度法测定。脂质过氧化程度通过血浆丙二醛（MDA）水平来评估。在对照组中，血浆脂质过氧化物的平均值为1.329±0.118 nmol/ml。在合并缺血性卒中的糖尿病患者中，MDA水平为2.919±0.182 nmol/l；p<0.001，在未合并缺血性卒中的患者中，MDA水平为2.329±0.149 nmol/l；p<0.05；合并缺血性卒中的糖尿病患者与未合并缺血性卒中的患者之间，p<0.01。高水平的脂质过氧化物可能引发一个自我维持的慢性过程，随着时间推移，可能导致糖尿病大血管和微血管病变的加重。过氧化氢酶的血浆水平采用Goth分光光度法测定。在114名健康人中，血浆过氧化氢酶（CAT）活性的平均值为115.3±14.5 MU/l，女性的血浆过氧化氢酶水平（108.7±12.4 MU/l）低于男性（118.9±16.6 MU/l）。合并缺血性卒中患者的血浆CAT平均值为（102.4±12.7 MU/l，p<0.001），未合并缺血性卒中患者的血浆CAT平均值为116.3±18.7 MU/l，p<0.05）；合并缺血性卒中的糖尿病患者与未合并缺血性卒中的患者之间，p<0.01。我们的结果显示，与未合并缺血性卒中的糖尿病患者相比，合并缺血性卒中的糖尿病患者血浆CAT活性降低。我们可以得出结论，糖尿病患者血浆CAT活性降低是氧化修饰的结果。这些结果表明，糖尿病患者的氧化损伤显著增加。
