Hartmann M, Kreuss M, Sommer K
Institute of Process Engineering of Disperse Systems, Life and Food Science Center, TU München, Freising, Germany.
Cell Mol Biol (Noisy-le-grand). 2004 Jun;50(4):479-84.
A high pressure chamber, which withstands a pressure up to 300 MPa has been developed. The so-called HPDS (Hartmann, Pfeifer, Dornheim, Sommer) High Pressure Cell in combination with an inverted microscope and an analysis system allows brilliant microscopic colour pictures with an optical resolution better than 0.56 microm. The pressure chamber allows the in situ observation of dynamic changes of microscopic structures in bright field, phase contrast and fluorescence microscopy. This publication should demonstrate the capabilities of the system using results of experiments with two types of Spirogyra algae. The pictures have shown significant variations of the chloroplasma and the cell wall membrane at pressures of up to 120 MPa. The new system provides a simple way to perform microscopic analyses at pressures of up to 300 MPa.
已经开发出一种能承受高达300兆帕压力的高压腔。所谓的HPDS（哈特曼、 Pfeifer、多恩海姆、索默）高压细胞与倒置显微镜和分析系统相结合，能够获得光学分辨率优于0.56微米的出色微观彩色图像。该压力腔允许在明场、相差和荧光显微镜下对微观结构的动态变化进行原位观察。本出版物应通过两种水绵藻的实验结果来展示该系统的功能。图片显示，在高达120兆帕的压力下，叶绿体和细胞壁膜有显著变化。新系统提供了一种在高达300兆帕的压力下进行微观分析的简单方法。
