Erlandsson Anna, Larsson Jimmy, Forsberg-Nilsson Karin
Department of Medical Biochemistry and Microbiology, Uppsala University, Biomedical Center, 751 23 Uppsala, Sweden.
Exp Cell Res. 2004 Dec 10;301(2):201-10. doi: 10.1016/j.yexcr.2004.08.009.
Migration of neural cells to their final positions is crucial for the correct formation of the central nervous system. Several extrinsic factors are known to be involved in the regulation of neural migration. We asked if stem cell factor (SCF), well known as a chemoattractant and survival factor in the hematopoietic lineage, could elicit similar responses in neural stem cells. For that purpose, a microchemotaxis assay was used to study the effect of SCF on migration of neural stem cells from the embryonic rat cortex. Our results show that SCF-induced chemotaxis and that specific antibodies to SCF or tyrosine kinase inhibitors abolished the migratory response. The SCF-receptor, Kit, was expressed in neural stem cells and in their differentiated progeny. We also show that SCF is a survival factor, but not a mitogen or a differentiation factor for neural stem cells. These data suggest a role for SCF in cell migration and survival in the developing cortex.
神经细胞迁移至其最终位置对于中枢神经系统的正确形成至关重要。已知有几种外在因素参与神经迁移的调控。我们探究了干细胞因子（SCF），它作为造血谱系中的趋化因子和存活因子广为人知，是否能在神经干细胞中引发类似反应。为此，采用微趋化性分析来研究SCF对胚胎大鼠皮质神经干细胞迁移的影响。我们的结果表明SCF诱导趋化作用，并且针对SCF的特异性抗体或酪氨酸激酶抑制剂消除了迁移反应。SCF受体Kit在神经干细胞及其分化后代中表达。我们还表明SCF是一种存活因子，但不是神经干细胞的促有丝分裂因子或分化因子。这些数据表明SCF在发育中的皮质细胞迁移和存活中发挥作用。
