鉴定在人类巨噬细胞中主要表达的基因。
Identification of genes predominantly expressed in human macrophages.
作者信息
Svensson Per-Arne, Hägg Daniel A, Jernås Margareta, Englund Mikael C O, Hulten Lillemor Mattsson, Ohlsson Bertil G, Hulthe Johannes, Wiklund Olov, Carlsson Björn, Fagerberg Björn, Carlsson Lena M S
机构信息
Department of Internal Medicine, Research Centre for Endocrinology and Metabolism, Vita Stråket 12, Sahlgrenska Academy, Göteborg University, S-41345 Göteborg, Sweden.
出版信息
Atherosclerosis. 2004 Dec;177(2):287-90. doi: 10.1016/j.atherosclerosis.2004.07.031.
Identification of cell and tissue specific genes may provide novel insights to signaling systems and functions. Macrophages play a key role in many diseases including atherosclerosis. Using DNA microarrays we compared the expression of approximately 10,000 genes in 56 human tissues and identified 23 genes with predominant expression in macrophages. The identified genes include both genes known to be macrophage specific and genes previously not well described in this cell type. Tissue distribution of two genes, liver X receptor (LXR) alpha and interleukin-1 receptor antagonist (IL1RN), was verified by real-time RT-PCR. We conclude that comparison of expression profiles from a large number of tissues can be used to identify genes that are predominantly expressed in certain tissues. Identification of novel macrophage specific genes may increase our understanding of the role of this cell in different diseases.
细胞和组织特异性基因的鉴定可能为信号系统和功能提供新的见解。巨噬细胞在包括动脉粥样硬化在内的许多疾病中起关键作用。我们使用DNA微阵列比较了56种人类组织中约10000个基因的表达，并鉴定出23个在巨噬细胞中主要表达的基因。所鉴定的基因包括已知为巨噬细胞特异性的基因和以前在这种细胞类型中未得到充分描述的基因。通过实时逆转录聚合酶链反应验证了两个基因，即肝X受体（LXR）α和白细胞介素-1受体拮抗剂（IL1RN）的组织分布。我们得出结论，对大量组织的表达谱进行比较可用于鉴定在某些组织中主要表达的基因。鉴定新的巨噬细胞特异性基因可能会增加我们对这种细胞在不同疾病中作用的理解。
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