Puente Patricia Fernández, Sáez María José Fidalgo, Hamilton Brett, Furey Ambrose, James Kevin J
PROTEOBIO, Mass Spectrometry Centre for Proteomics and Biotoxin Research, Department of Chemistry,Cork Institute of Technology, Bishopstown, Cork, Ireland.
Toxicon. 2004 Dec 15;44(8):919-26. doi: 10.1016/j.toxicon.2004.09.001.
Diarretic shellfish poisoning (DSP) is a toxic syndrome associated with the consumption of bivalve molluscs. The DSP toxins are polyether compounds, which include okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs) and pectenotoxin seco acids (PTX2SAs). These toxins originate in marine dinoflagellates, including Dinophysis spp. Phytoplankton samples were collected from the southwest coast of Ireland and D. acuta was the predominant species. Monocultures of D. acuta cells were prepared by hand picking from microscope slides in order to confirm their toxin profiles. There was a remarkable consistency in the toxin profiles in all of the phytoplankton samples collected during the summer months, irrespective of location, depth or mesh size. Analysis using liquid chromatography-multiple tandem mass spectrometry (LC-MS/MS) revealed that DTX2 and OA were the predominant toxins at a consistent ratio. The average toxin composition was: DTX2 (53+/-5%), OA (26.5+/-2.3%) and total pectenotoxins (20.8+/-4.7%). Toxin profiles in D. acuta from Europe were distinctly different from those found in New Zealand, where PTX2 was the predominant toxin and DTX2 was absent.
腹泻性贝类中毒（DSP）是一种与食用双壳贝类有关的中毒综合征。DSP毒素是多醚化合物，包括冈田酸（OA）、鳍藻毒素（DTXs）、pectenotoxins（PTXs）和pectenotoxin seco acids（PTX2SAs）。这些毒素起源于海洋甲藻，包括鳍藻属。从爱尔兰西南海岸采集浮游植物样本，其中尖鳍藻是优势种。通过从显微镜载玻片上手工挑选制备尖鳍藻细胞的单培养物，以确认其毒素谱。在夏季采集的所有浮游植物样本中，无论位置、深度或网目尺寸如何，毒素谱都有显著的一致性。使用液相色谱-多反应监测串联质谱（LC-MS/MS）分析表明，DTX2和OA是主要毒素，且比例一致。平均毒素组成是：DTX2（53±5%）、OA（26.5±2.3%）和总pectenotoxins（20.8±4.7%）。欧洲尖鳍藻的毒素谱与新西兰明显不同，在新西兰PTX2是主要毒素且不存在DTX2。
