Ahn Meejung, Lee Yongduk, Moon Changjong, Jin Jae-Kwang, Matsumoto Yoh, Koh Chang-Sung, Kim Hyung-Min, Shin Taekyun
Department of Veterinary Medicine, Cheju National University, Jeju 690-756, South Korea.
Neurosci Lett. 2004 Nov 30;372(1-2):137-41. doi: 10.1016/j.neulet.2004.09.028.
We examined the expression of osteopontin (OPN) in the sciatic nerves of rats with experimental autoimmune neuritis (EAN), using immunohistochemistry and immunoblotting, to study its involvement in the pathogenesis of autoimmune peripheral nervous system diseases. Constitutive OPN expression was detected in some Schwann cells; expression was increased after immunization with adjuvant alone. At day 14 after induction of EAN, many Schwann cells had a granular pattern of immunoreactivity, whereas very few inflammatory cells were OPN-positive. Even after recovery from hindlimb paralysis, at 24 days post-immunization, OPN expression remained elevated in the Schwann cells. The results suggest that OPN expression in Schwann cells is easily induced by immunostimulation, and further enhanced by the inflammatory reaction in EAN. Continued elevation of OPN after recovery may represent a functional recovery after a transient inflammatory insult.
我们运用免疫组织化学和免疫印迹法检测了实验性自身免疫性神经炎（EAN）大鼠坐骨神经中骨桥蛋白（OPN）的表达，以研究其在自身免疫性周围神经系统疾病发病机制中的作用。在一些施万细胞中检测到组成型OPN表达；单独用佐剂免疫后表达增加。在诱导EAN后第14天，许多施万细胞呈现颗粒状免疫反应模式，而很少有炎性细胞呈OPN阳性。即使在后肢麻痹恢复后，免疫后24天，施万细胞中OPN表达仍保持升高。结果表明，施万细胞中的OPN表达容易被免疫刺激诱导，并在EAN的炎症反应中进一步增强。恢复后OPN持续升高可能代表短暂炎症损伤后的功能恢复。
