Baró I, Eisner D A
Department of Veterinary Preclinical Sciences, University of Liverpool, UK.
Pflugers Arch. 1992 Jan;420(1):115-7. doi: 10.1007/BF00378652.
The effects of thapsigargin were studied on single cells isolated from side branches of the rat mesenteric artery. Thapsigargin (150 nM) produced a transient increase of [Ca2+]i. This transient rise of [Ca2+]i was unaffected by removing external Ca2+ ions. This suggests that thapsigargin is releasing Ca2+ ions from an intracellular store. In the absence of thapsigargin both noradrenaline and caffeine also produced a transient increase of [Ca2+]i. These increases were abolished by prior exposure to thapsigargin. Correspondingly, the effects of thapsigargin were abolished by prior exposure to caffeine. These results show that thapsigargin releases Ca2+ from the noradrenaline and caffeine-sensitive stores.
研究了毒胡萝卜素对从大鼠肠系膜动脉侧支分离出的单个细胞的影响。毒胡萝卜素（150 nM）使细胞内钙离子浓度（[Ca2+]i）短暂升高。去除细胞外钙离子后，这种[Ca2+]i的短暂升高不受影响。这表明毒胡萝卜素是从细胞内储存库释放钙离子。在没有毒胡萝卜素的情况下，去甲肾上腺素和咖啡因也会使[Ca2+]i短暂升高。预先暴露于毒胡萝卜素会消除这些升高。相应地，预先暴露于咖啡因会消除毒胡萝卜素的作用。这些结果表明，毒胡萝卜素从对去甲肾上腺素和咖啡因敏感的储存库中释放钙离子。
