缓激肽B2受体拮抗剂对犬肝脏延长切除并缺血的影响。
The effects of a bradykinin B2 receptor antagonist in extended liver resection with ischemia in dogs.
作者信息
Totsuka Osamu, Takeyoshi Izumi, Tsutsumi Hirofumi, Hashimoto Naoki, Sunose Yutaka, Tokumine Masahiko, Ohwada Susumu, Matsumoto Koshi, Morishita Yasuo
机构信息
Second Department of Surgery, Gunma University School of Medicine, Maebashi, Gunma, Japan.
出版信息
Hepatogastroenterology. 2004 Nov-Dec;51(60):1771-5.
BACKGROUND/AIMS: In liver surgery, total clamping of the portal triad (Pringle's procedure) is commonly used, and sometimes causes liver failure. This study evaluated the effects of a bradykinin B2 receptor antagonist, FR173657 (FR), on ischemia-reperfusion injury during liver resection in dogs.
METHODOLOGY
Experimental animals were divided into two groups. In the FR group (n=6), FR (100 nmol/kg/hr) was administered continuously via the portal vein from 30 min before the onset of ischemia until 2 hr after reperfusion. In the control group (n=6), vehicle was injected in the same manner. The right portal pedicle was clamped for 60 min, while the left portal branch was left patent to avoid portal congestion. Following reperfusion, the non-ischemic lobes were resected, and remnant liver function was evaluated.
RESULTS
AST and ALT were significantly (p<0.05) lower in the FR group than in the control group. Hepatic tissue blood flow 30 min after reperfusion was significantly (p<0.05) higher in the FR group than in the control group. Histological tissue damage was mild, and polymorphonuclear neutrophil infiltration was significantly (p<0.05) reduced in the FR group compared with the control group.
CONCLUSIONS
A bradykinin B2 receptor antagonist ameliorated the ischemia-reperfusion injury caused by Pringle's procedure during extended liver resection.
背景/目的：在肝脏手术中，门静脉三联征的完全阻断（普林格尔手术）被广泛应用，但有时会导致肝衰竭。本研究评估了缓激肽B2受体拮抗剂FR173657（FR）对犬肝切除术中缺血再灌注损伤的影响。
方法
将实验动物分为两组。FR组（n = 6），从缺血开始前30分钟至再灌注后2小时，通过门静脉持续给予FR（100 nmol/kg/小时）。对照组（n = 6），以相同方式注射溶剂。右门静脉蒂夹闭60分钟，而左门静脉分支保持开放以避免门静脉淤血。再灌注后，切除非缺血叶，并评估残余肝功能。
结果
FR组的AST和ALT显著低于对照组（p < 0.05）。再灌注30分钟后，FR组的肝组织血流量显著高于对照组（p < 0.05）。组织学检查显示组织损伤较轻，与对照组相比，FR组的多形核中性粒细胞浸润显著减少（p < 0.05）。
结论
缓激肽B2受体拮抗剂可改善扩大肝切除术中普林格尔手术引起的缺血再灌注损伤。
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