Totsuka Osamu, Takeyoshi Izumi, Tsutsumi Hirofumi, Arakawa Kazuhisa, Akao Takahiko, Muraoka Masato, Ohwada Susumu, Matsumoto Koshi, Morishita Yasuo
Second Department of Surgery, Gunma University School of Medicine, Gunma, Japan.
Hepatogastroenterology. 2005 Sep-Oct;52(65):1545-8.
BACKGROUND/AIMS: In liver surgery, total clamping of the portal triad (Pringle's procedure) is commonly used, and this sometimes causes liver failure. This study evaluated the effects of a free radical scavenger, MCI-186, on ischemia-reperfusion injury during liver resection in dogs.
The experimental animals were divided into two groups. In the MCI group (n = 6), MCI-186 (6mg/kg/h) was administered twice, through a catheter placed in the right hepatic vein: the first time was from 0.5 hours before the onset of ischemia until ischemia by partial inflow occlusion, and the second was from 0.5-hours before reperfusion until reperfusion. In the control group (n = 6), vehicle (physiological saline) was administered in the same manner.
The serum AST, ALT, and LDH levels were significantly (P < 0.05) lower in the MCI group than in the control group. Hepatic tissue blood flow 0.5 hours after reperfusion was significantly (P < 0.05) higher in the MCI group than in the control group. Histological tissue damage was mild, and tissue MDA levels were significantly (P < 0.05) lower in the MCI group than in the control group.
MCI-186 ameliorates the ischemia-reperfusion injury caused by Pringle's procedure during extended liver resection.
背景/目的：在肝脏手术中，门静脉三联征完全阻断（普林格尔手术）被广泛应用，但有时会导致肝衰竭。本研究评估了自由基清除剂MCI-186对犬肝切除术中缺血再灌注损伤的影响。
将实验动物分为两组。MCI组（n = 6）通过置于右肝静脉的导管给予MCI-186（6mg/kg/h）两次：第一次在缺血开始前0.5小时至部分入流阻断导致缺血时，第二次在再灌注前0.5小时至再灌注时。对照组（n = 6）以相同方式给予溶媒（生理盐水）。
MCI组血清AST、ALT和LDH水平显著低于对照组（P < 0.05）。再灌注0.5小时后，MCI组肝组织血流量显著高于对照组（P < 0.05）。组织学上组织损伤较轻，MCI组组织MDA水平显著低于对照组（P < 0.05）。
MCI-186可改善扩大肝切除术中普林格尔手术所致的缺血再灌注损伤。
