Disch Jolande, Oliveira Maria Cláudia, Orsini Marcela, Rabello Ana
Laboratório de Pesquisas Clínicas, Centro de Pesquisas René Rachou-FIOCRUZ. Av. Augusto de Lima, 1715, 30190-002, Belo Horizonte, MG, Brazil.
Acta Trop. 2004 Nov-Dec;92(3):279-83. doi: 10.1016/j.actatropica.2004.08.002.
With the aim of evaluating the utility of the detection of Leishmania kDNA in peripheral blood for the cure assessment of visceral leishmaniasis (VL), a PCR based method was performed in patients with confirmed VL at three follow-up periods after specific chemotherapy with pentavalent antimonial. In 16 out of 17 (94.1%) patients with pre-treatment detectable kDNA that were clinically cured, the PCR turned negative up to 37 days after the initiation of treatment, remaining negative over 90 days after treatment. The clearance of Leishmania kDNA from peripheral blood of patients with VL hints to occur during or shortly after treatment concurring or preceding clinical recovery.
为了评估检测外周血中利什曼原虫kDNA在内脏利什曼病（VL）治愈评估中的效用，对确诊为VL的患者在使用五价锑进行特异性化疗后的三个随访期采用了基于PCR的方法。在17例治疗前可检测到kDNA且临床治愈的患者中，有16例（94.1%）在治疗开始后37天内PCR转为阴性，治疗后90天以上仍为阴性。VL患者外周血中利什曼原虫kDNA的清除似乎发生在治疗期间或治疗后不久，与临床恢复同时或先于临床恢复。
