Li Ge, Kim Dong-Hee, Kim Tae-Dong, Park Byoung-Jeon, Park Hae-Duck, Park Jong-Il, Na Min-Kyun, Kim Hwan-Chul, Hong Nam-Doo, Lim Kyu, Hwang Byung-Doo, Yoon Wan-Hee
Department of Surgery ,College of Medicine, Cancer Research Institute, Chungnam National University and Hospital, 640 Daesa-Dong, Joong-Ku, Daejon 301-721, South Korea.
Cancer Lett. 2004 Dec 28;216(2):175-81. doi: 10.1016/j.canlet.2004.07.014.
The cytotoxic mechanism of protein-bound polysaccharide isolated from Phellinus linteus (PL, Mesima) has been investigated. PL inhibited the proliferation and colony formation of SW480 human colon cancer cells. Flow cytometry analysis showed that PL increased the populations of both apoptotic sub-G1 and G2/M phase. The result obtained from TUNEL assay corroborated apoptosis which was shown in flow cytometry. Western blot analysis suggested that PL-induced apoptosis and growth inhibition were associated with decrease in Bcl-2, increase of the release of cytochrome c, and reduced expression of cyclin B1. These results suggest that PL has a direct antitumor effect through apoptosis and cell cycle blockade in certain cancer cells.
对从桑黄（PL，桑黄）中分离出的蛋白结合多糖的细胞毒性机制进行了研究。桑黄抑制了SW480人结肠癌细胞的增殖和集落形成。流式细胞术分析表明，桑黄增加了凋亡亚G1期和G2/M期的细胞群体。TUNEL检测结果证实了流式细胞术中显示的细胞凋亡。蛋白质印迹分析表明，桑黄诱导的细胞凋亡和生长抑制与Bcl-2减少、细胞色素c释放增加以及细胞周期蛋白B1表达降低有关。这些结果表明，桑黄通过诱导某些癌细胞凋亡和细胞周期阻滞具有直接抗肿瘤作用。
