Ertas O S, Tezel H
Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, 35100 Bornova, Izmir, Turkey.
J Pharm Biomed Anal. 2004 Nov 19;36(4):893-7. doi: 10.1016/j.jpba.2004.08.014.
A cold vapour-atomic absorption spectrometry (CV-AAS) method for determining mercury in dental students and clinical teaching staff red blood cells at a dental school using amalgam as a restorative material has been validated. A number of blood samples (n = 122) from dental students in years I to V, clinical teachers in restorative dentistry and controls were collected and analysed. Accuracy, linearity, precision (repeatability and reproducibility) and robustness of the method have been determined, and detection and quantification limits have been calculated. Linearity of response was verified for concentrations ranging from 5 to 40 microgL(-1) of mercury. Correlation coefficient of the calibration straight lines was always >/=0.99. Intra-day precision of the method gave coefficient of variation (CV) of 5.51%. Inter-day precision of the method calculated after analysis of five different concentrations of mercury standard solutions by the same analyst in different days and by two different analysts in different days gave coefficient of variation 4.89 and 5.44%, respectively. The accuracy of the method was calculated a CRM NIST 966 (toxic metals in bovine blood) total amount of mercury was found a concentration of 28.83 +/- 2.2 microgL(-1). Recovery was 89.27%. Robustness of the method evaluated by changing different experimental conditions under which analyses performed, fractional factorial design was done for assessing robustness of the method. Root mean square error was found out as 1.56. Limits of detection and quantification were 1.84 and 4.03 microg of Hg per litre of sample, respectively. Results show the suitability of the method for direct measurement of mercury in red blood cells and the importance of the working conditions for people dealing with amalgam at a dental school.
一种冷蒸气原子吸收光谱法（CV-AAS）已得到验证，该方法用于测定一所牙科学校中使用汞合金作为修复材料的牙科学生和临床教师红细胞中的汞含量。收集并分析了来自一至五年级牙科学生、修复牙科临床教师以及对照组的若干血液样本（n = 122）。已确定了该方法的准确性、线性、精密度（重复性和再现性）及稳健性，并计算了检测限和定量限。在汞浓度为5至40 μg/L的范围内验证了响应的线性。校准直线的相关系数始终≥0.99。该方法的日内精密度给出的变异系数（CV）为5.51%。由同一位分析人员在不同日期以及两位不同分析人员在不同日期对五种不同浓度的汞标准溶液进行分析后计算得出的日间精密度，变异系数分别为4.89%和5.44%。通过CRM NIST 966（牛血中的有毒金属）计算该方法的准确性，发现汞的总量浓度为28.83±2.2 μg/L。回收率为89.27%。通过改变进行分析的不同实验条件来评估该方法的稳健性，采用分数因子设计来评估该方法的稳健性。得出均方根误差为1.56。检测限和定量限分别为每升样品中1.84 μg和4.03 μg的汞。结果表明该方法适用于直接测量红细胞中的汞，以及工作条件对牙科学校中接触汞合金人员的重要性。
