Braunschweig Martin H, Van Laere Anne-Sophie, Buys Nadine, Andersson Leif, Andersson Göran
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 597, SE-751 24 Uppsala, Sweden.
Genomics. 2004 Dec;84(6):1021-9. doi: 10.1016/j.ygeno.2004.09.006.
A paternally expressed quantitative trait locus (QTL) for muscle mass was mapped to the insulin-like growth factor II (IGF2) locus at the distal end of pig chromosome 2p. It was recently demonstrated that a G to A transition at position intron 3-nt 3072 is the quantitative trait nucleotide (QTN) underlying this QTL. In this study we report for the first time the existence of imprinted porcine IGF2 antisense transcripts (IGF2-AS) and demonstrate that their expression in postnatal muscle is also affected by the QTN. A coregulated expression of IGF2 and IGF2-AS RNAs in muscle and liver with decreasing transcription from fetal to adult age was demonstrated. Further, the significant difference found in expression of IGF2-AS in postnatal muscle between individuals with different QTL genotypes and the lack of significant differences in fetal muscle and liver reflect completely what has been found for IGF2 sense transcripts.
一个父系表达的肌肉质量数量性状位点（QTL）被定位到猪2号染色体短臂远端的胰岛素样生长因子II（IGF2）基因座。最近证明，内含子3第3072位核苷酸处的G到A转换是该QTL的数量性状核苷酸（QTN）。在本研究中，我们首次报道了印记猪IGF2反义转录本（IGF2-AS）的存在，并证明它们在出生后肌肉中的表达也受QTN影响。IGF2和IGF2-AS RNA在肌肉和肝脏中的表达呈共调节，从胎儿期到成年期转录逐渐减少。此外，不同QTL基因型个体出生后肌肉中IGF2-AS表达的显著差异以及胎儿肌肉和肝脏中无显著差异，完全反映了IGF2正义转录本的情况。
