Paul Shyam Sunder, Kamra Devki Nandan, Sastry Vadali Rama Bhadra, Sahu Narottam Prasad, Agarwal Neeta
Central Institute for Research on Buffaloes, Nabha 147201, Patiala, India.
Reprod Nutr Dev. 2004 Jul-Aug;44(4):313-9. doi: 10.1051/rnd:2004036.
Five strains of anaerobic fungi isolated from the faeces of wild (hog deer, Cervus porcinus; blackbuck, Antelope cervicapra; spotted deer, Axis axis; nilgai, Baselophus tragocamelus) and rumen liquor of domestic (sheep, Ovies aries) ruminants showing high fibrolytic enzyme producing ability were added to mixed rumen microflora of buffalo to study their effect on the digestibility of lignocellulosic feed (wheat straw and wheat bran in the ratio of 80:20), enzyme production and fermentation end products in in vitro conditions. Among the 5 isolates studied, FNG5 (isolated from nilgai) showed the highest stimulating effect on apparent digestibility (35.31 +/- 1.61% vs. 28.61 +/- 1.55%; P < 0.05), true digestibility (43.64 +/- 1.73% vs. 35.37 +/- 1.65%; P < 0.01), neutral detergent fiber digestibility (29.30 +/- 2.58% vs. 18.47 +/- 2.12; P < 0.01) of feed 24 h after inoculation compared to the control group. The production of carboxymethyl cellulase, xylanase, acetyl esterase and beta-glucosidase was significantly (P < 0.05) higher in the FNG5 inoculated incubation medium. There was no improvement in the digestibility and enzyme production on the addition of the other 4 isolates. Total volatile fatty acid levels as well as the concentration of acetate, propionate, isobutyrate and valerate were significantly higher in the FNG5 added group as compared to the control group. The fungal isolate FNG5 from nilgai, a wild ruminant, was found to be superior to the other isolates tested and appears to have a potential to be used as a feed additive for improving fiber degradation in domestic ruminants.
从野生反刍动物（豚鹿、黑羚、花鹿、牛羚）粪便以及家养反刍动物（绵羊）瘤胃液中分离出5株具有高纤维分解酶产生能力的厌氧真菌，将其添加到水牛的混合瘤胃微生物群中，以研究它们在体外条件下对木质纤维素饲料（小麦秸秆和麦麸比例为80:20）消化率、酶产生及发酵终产物的影响。在所研究的5株分离菌株中，FNG5（从牛羚分离得到）对接种24小时后饲料的表观消化率（35.31±1.61%对28.61±1.55%；P<0.05）、真消化率（43.64±1.73%对35.37±1.65%；P<0.01）、中性洗涤纤维消化率（29.30±2.58%对18.47±2.12；P<0.01）显示出最高的刺激作用。与对照组相比，接种FNG5的培养基中羧甲基纤维素酶、木聚糖酶、乙酰酯酶和β-葡萄糖苷酶的产生显著（P<0.05）更高。添加其他4株分离菌株后，消化率和酶产生没有改善。与对照组相比，添加FNG5的组总挥发性脂肪酸水平以及乙酸、丙酸、异丁酸和戊酸的浓度显著更高。发现来自野生反刍动物牛羚的真菌分离株FNG5优于其他测试分离株，似乎有潜力用作饲料添加剂以改善家养反刍动物的纤维降解。
