Betz W J, Bewick G S
Department of Physiology, University of Colorado School of Medicine, Denver 80262.
Science. 1992 Jan 10;255(5041):200-3. doi: 10.1126/science.1553547.
The fluorescent dyes FM1-43 and RH414 label motor nerve terminals in an activity-dependent fashion that involves dye uptake by synaptic vesicles that are recycling. This allows optical monitoring of vesicle recycling in living nerve terminals to determine how recycled vesicles reenter the vesicle pool. The results suggest that recycled vesicles mix with the pool morphologically and functionally. One complete cycle of release of transmitter, recycling of a vesicle, and rerelease of transmitter appears to take about 1 minute.
荧光染料FM1-43和RH414以一种依赖于活性的方式标记运动神经末梢，这种方式涉及通过正在循环的突触小泡摄取染料。这使得能够对活神经末梢中的小泡循环进行光学监测，以确定循环后的小泡如何重新进入小泡池。结果表明，循环后的小泡在形态和功能上与小泡池混合。递质释放、小泡循环以及递质再次释放的一个完整周期似乎大约需要1分钟。
