Devillers Nicolas, Farmer Chantal, Mounier Anne-Marie, Le Dividich Jean, Prunier Armelle
Institut National de la Recherche Agronomique, Unité Mixte de Recherche sur le Veau et le Porc, 35590 Saint-Gilles, France.
Reprod Nutr Dev. 2004 Jul-Aug;44(4):381-96. doi: 10.1051/rnd:2004043.
Blood, colostrum and saliva samples were serially taken from 6 multiparous sows from day 109 of gestation until day 3 postpartum. Plasma was assayed for oestradiol-17beta (E2), progesterone (P4), prolactin (PRL), cortisol, immunoglobulin G (IgG) and lactose. Colostrum was assayed for E2, P4, IgG and lactose. Lactoserum, obtained after ultra centrifugation of colostrum, was assayed for PRL. Saliva was assayed for cortisol. Time-related variations in hormone, IgG and lactose concentrations measured in plasma were parallel to those measured in colostrum, lactoserum or saliva. However, the concentrations were higher in colostrum or lactoserum and lower in saliva than in plasma. Ratios of concentrations of cortisol in saliva and PRL in lactoserum over those in plasma did not vary with time and averaged 0.2 and 1.6, respectively. Conversely, the ratios of concentrations of E2 and P4 in colostrum over those in plasma varied with time (P < 0.05) but were quite constant before the end of parturition, averaging 2.7 and 3.6, respectively. The ratios of concentrations of IgG and lactose in colostrum over those in plasma also varied with time (P < 0.05). The concentrations of hormones in plasma on the one hand and in colostrum, lactoserum or saliva on the other hand were significantly correlated but correlations varied with time (PRL across periods: r = 0.31; cortisol across periods: r = 0.60; E2 during parturition: r = 0.83; P4 before parturition: r = 0.82; P4 during parturition: r = 0.67). The present results indicate that around parturition, assays of hormones in colostrum or saliva can be used to study the hormonal status of sows. Furthermore, variations in colostrum and plasma concentrations of IgG and lactose are good indicators of the transition from colostrum to milk synthesis.
从妊娠第109天至产后第3天，连续采集6头经产母猪的血液、初乳和唾液样本。检测血浆中的雌二醇-17β（E2）、孕酮（P4）、催乳素（PRL）、皮质醇、免疫球蛋白G（IgG）和乳糖。检测初乳中的E2、P4、IgG和乳糖。初乳经超速离心后获得的乳清，检测其中的PRL。检测唾液中的皮质醇。血浆中激素、IgG和乳糖浓度随时间的变化与初乳、乳清或唾液中检测到的变化平行。然而，初乳或乳清中的浓度高于血浆，而唾液中的浓度低于血浆。唾液中皮质醇浓度与乳清中PRL浓度与血浆中相应浓度的比值不随时间变化，平均分别为0.2和1.6。相反，初乳中E2和P4浓度与血浆中相应浓度的比值随时间变化（P<0.05），但在分娩结束前相当稳定，平均分别为2.7和3.6。初乳中IgG和乳糖浓度与血浆中相应浓度的比值也随时间变化（P<0.05）。一方面血浆中的激素浓度与另一方面初乳、乳清或唾液中的激素浓度显著相关，但相关性随时间变化（各时期PRL：r=0.31；各时期皮质醇：r=0.60；分娩期间E2：r=0.83；分娩前P4：r=0.82；分娩期间P4：r=0.67）。目前的结果表明，在分娩前后，检测初乳或唾液中的激素可用于研究母猪的激素状态。此外，初乳和血浆中IgG和乳糖浓度的变化是初乳向乳汁合成转变的良好指标。
