Kittayapong P, Baisley K J, Baimai V, O'Neill S L
Department of Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
J Med Entomol. 2000 May;37(3):340-5. doi: 10.1093/jmedent/37.3.340.
Wolbachia are a group of intracellular inherited bacteria that infect a wide range of arthropods. They are associated with a variety of reproductive alterations in their hosts, the best known being cytoplasmic incompatability. The Wolbachia pipientis assemblage has been divided into two major groups (A and B) and 12 subgroups. We report herein the first systematic survey of Wolbachia in mosquitoes, and the first survey classifying Wolbachia infections by subgroup. Wolbachia were detected in 28.1% of 89 wild-caught mosquito species, based on a polymerase chain reaction assay using ftsZ and wsp gene primers. Infections were found in all major disease vector genera except Anopheles. Nine of the 12 Wolbachia subgroups were represented. Group B Wolbachia strains showed more phylogenetic concordance with their host taxa than group A strains. Of the 25 positive mosquito species, five were superinfected with group A bacteria strains (AA), eight were superinfected with A and B strains (AB), and one was superinfected with group B strains (BB). The widespread distribution of Wolbachia among mosquito species further supports their potential importance in the genetic control of disease vectors.
沃尔巴克氏体是一类细胞内寄生的遗传细菌，可感染多种节肢动物。它们与宿主的多种生殖改变有关，其中最著名的是细胞质不亲和。嗜虫沃尔巴克氏体已被分为两个主要组（A组和B组）以及12个亚组。我们在此报告了对蚊子中沃尔巴克氏体的首次系统调查，以及首次按亚组对沃尔巴克氏体感染进行分类的调查。基于使用ftsZ和wsp基因引物的聚合酶链反应分析，在89种野生捕获的蚊子中，有28.1%检测到了沃尔巴克氏体。在除按蚊属之外的所有主要病媒属中均发现了感染。12个沃尔巴克氏体亚组中有9个有代表。与A组菌株相比，B组沃尔巴克氏体菌株与其宿主分类群在系统发育上表现出更高的一致性。在25种呈阳性的蚊子中，有5种被A组细菌菌株双重感染（AA），8种被A组和B组菌株双重感染（AB），1种被B组菌株双重感染（BB）。沃尔巴克氏体在蚊子物种中的广泛分布进一步证明了它们在病媒遗传控制中的潜在重要性。
