从已知或疑似鼠疫活动地区采集的跳蚤（蚤目：蚤科、角叶蚤科和鬃眼蚤科）中的鼠疫耶尔森菌数量。
Quantities of Yersinia pestis in fleas (Siphonaptera: Pulicidae, Ceratophyllidae, and Hystrichopsyllidae) collected from areas of known or suspected plague activity.
作者信息
Engelthaler D M, Gage K L
机构信息
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, P.O. Box 2087, Fort Collins, CO 80522, USA.
出版信息
J Med Entomol. 2000 May;37(3):422-6. doi: 10.1093/jmedent/37.3.422.
We used a quantitative competitive polymerase chain reaction (PCR) (QC-PCR) to determine bacterial loads in 669 fleas collected in areas of confirmed and suspected plague epizootics. Fleas were collected out of rodent burrows (67.9%) and off of captured animals (24.1%) and rodent carcasses (8.1%). An initial PCR screening assay indicated that 12.1% (81/669) of all fleas were positive for Yersinia pestis. Fleas collected from burrows had significantly lower (chi2 = 264.9, P < 0.0001) infection rates (6.8%) but significantly higher (Student t-test, P < 0.0001) bacterial loads (mean = 10(5.6) Y. pestis per flea) than fleas collected off of rodent carcasses (infection rate = 92.6%; mean bacterial load = 10(4.8) Y. pestis per flea). None of the fleas collected off of captured animals were positive for Y. pestis by PCR, although seven of the 176 captured animals were serologically positive for Y. pestis.
我们采用定量竞争聚合酶链反应（PCR）（QC-PCR）来测定在已确诊和疑似鼠疫动物流行病区域采集的669只跳蚤中的细菌载量。跳蚤是从鼠洞（67.9%）、捕获的动物身上（24.1%）以及鼠类尸体上（8.1%）采集的。初步的PCR筛选检测表明，所有跳蚤中有12.1%（81/669）对鼠疫耶尔森菌呈阳性。从鼠洞采集的跳蚤感染率显著较低（χ2 = 264.9，P < 0.0001）（6.8%），但细菌载量显著较高（学生t检验，P < 0.0001）（平均每只跳蚤有10(5.6)个鼠疫耶尔森菌），高于从鼠类尸体上采集的跳蚤（感染率 = 92.6%；平均细菌载量 = 10(4.8)个鼠疫耶尔森菌/只跳蚤）。通过PCR检测，从捕获动物身上采集的跳蚤中没有一只对鼠疫耶尔森菌呈阳性，尽管176只捕获动物中有7只血清学检测对鼠疫耶尔森菌呈阳性。
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