Lima José Bento Pereira, Valle Denise, Peixoto Alexandre Afranio
Departments of Entomology and Biochemistry and Molecular Biology, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ 21045-900, Brazil.
J Med Entomol. 2004 Sep;41(5):888-93. doi: 10.1603/0022-2585-41.5.888.
Complexes of sibling species are common among mosquitoes, and their existence within vector species can have important epidemiological consequences. Anopheles albitarsis sensu stricto and Anopheles deaneorum Rosa-Freitas are two putative vectors of malaria parasites belonging to the Albitarsis species complex (Diptera: Culicidae). Using an induced mating technique, we studied the reproductive isolation between these two closely related species and their reciprocal hybrids. Evidence for hybrid male sterility consistent with Haldane's rule was found. The results indicate that male hybrids show very low insemination rates, probably due to abnormalities in their reproductive organs. In addition, the data show that hybrid males carrying an X chromosome derived from An. deaneorum perform significantly worse than hybrid males carrying an An. albitarsis s.s. X chromosome.
同胞物种复合体在蚊子中很常见，它们在病媒物种中的存在可能会产生重要的流行病学后果。狭义的白跗按蚊和迪氏按蚊是属于白跗按蚊物种复合体（双翅目：蚊科）的两种假定的疟原虫病媒。我们使用诱导交配技术研究了这两个近缘物种及其正反交杂种之间的生殖隔离。发现了与霍尔丹法则一致的杂种雄性不育的证据。结果表明，杂种雄性的授精率非常低，这可能是由于其生殖器官异常所致。此外，数据显示，携带源自迪氏按蚊的X染色体的杂种雄性比携带狭义白跗按蚊X染色体的杂种雄性表现明显更差。
